Cargando…

Red fluorescent biofilm: the thick, the old, and the cariogenic

BACKGROUND: Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown. OBJECTIVE: The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms. DESIGN: T...

Descripción completa

Detalles Bibliográficos
Autores principales: Volgenant, Catherine M.C., Hoogenkamp, Michel A., Buijs, Mark J., Zaura, Egija, ten Cate, Jacob (Bob) M., van der Veen, Monique H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826460/
https://www.ncbi.nlm.nih.gov/pubmed/27060056
http://dx.doi.org/10.3402/jom.v8.30346
_version_ 1782426337109082112
author Volgenant, Catherine M.C.
Hoogenkamp, Michel A.
Buijs, Mark J.
Zaura, Egija
ten Cate, Jacob (Bob) M.
van der Veen, Monique H.
author_facet Volgenant, Catherine M.C.
Hoogenkamp, Michel A.
Buijs, Mark J.
Zaura, Egija
ten Cate, Jacob (Bob) M.
van der Veen, Monique H.
author_sort Volgenant, Catherine M.C.
collection PubMed
description BACKGROUND: Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown. OBJECTIVE: The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms. DESIGN: The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λ(exc)405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene. RESULTS: From day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence. CONCLUSIONS: The current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence.
format Online
Article
Text
id pubmed-4826460
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Co-Action Publishing
record_format MEDLINE/PubMed
spelling pubmed-48264602016-04-29 Red fluorescent biofilm: the thick, the old, and the cariogenic Volgenant, Catherine M.C. Hoogenkamp, Michel A. Buijs, Mark J. Zaura, Egija ten Cate, Jacob (Bob) M. van der Veen, Monique H. J Oral Microbiol Original Article BACKGROUND: Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown. OBJECTIVE: The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms. DESIGN: The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λ(exc)405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene. RESULTS: From day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence. CONCLUSIONS: The current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence. Co-Action Publishing 2016-04-07 /pmc/articles/PMC4826460/ /pubmed/27060056 http://dx.doi.org/10.3402/jom.v8.30346 Text en © 2016 Catherine M.C. Volgenant et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Volgenant, Catherine M.C.
Hoogenkamp, Michel A.
Buijs, Mark J.
Zaura, Egija
ten Cate, Jacob (Bob) M.
van der Veen, Monique H.
Red fluorescent biofilm: the thick, the old, and the cariogenic
title Red fluorescent biofilm: the thick, the old, and the cariogenic
title_full Red fluorescent biofilm: the thick, the old, and the cariogenic
title_fullStr Red fluorescent biofilm: the thick, the old, and the cariogenic
title_full_unstemmed Red fluorescent biofilm: the thick, the old, and the cariogenic
title_short Red fluorescent biofilm: the thick, the old, and the cariogenic
title_sort red fluorescent biofilm: the thick, the old, and the cariogenic
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826460/
https://www.ncbi.nlm.nih.gov/pubmed/27060056
http://dx.doi.org/10.3402/jom.v8.30346
work_keys_str_mv AT volgenantcatherinemc redfluorescentbiofilmthethicktheoldandthecariogenic
AT hoogenkampmichela redfluorescentbiofilmthethicktheoldandthecariogenic
AT buijsmarkj redfluorescentbiofilmthethicktheoldandthecariogenic
AT zauraegija redfluorescentbiofilmthethicktheoldandthecariogenic
AT tencatejacobbobm redfluorescentbiofilmthethicktheoldandthecariogenic
AT vanderveenmoniqueh redfluorescentbiofilmthethicktheoldandthecariogenic