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Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.

Methyl jasmonate (MJ) is an important plant growth regulator, involved in plant defense against abiotic stresses, however, its possible function in response to metal stress is poorly understood. In the present study, the effect of MJ on physiological and biochemical changes of the plants exposed to...

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Autores principales: Farooq, Muhammad A., Gill, Rafaqat A., Islam, Faisal, Ali, Basharat, Liu, Hongbo, Xu, Jianxiang, He, Shuiping, Zhou, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826882/
https://www.ncbi.nlm.nih.gov/pubmed/27148299
http://dx.doi.org/10.3389/fpls.2016.00468
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author Farooq, Muhammad A.
Gill, Rafaqat A.
Islam, Faisal
Ali, Basharat
Liu, Hongbo
Xu, Jianxiang
He, Shuiping
Zhou, Weijun
author_facet Farooq, Muhammad A.
Gill, Rafaqat A.
Islam, Faisal
Ali, Basharat
Liu, Hongbo
Xu, Jianxiang
He, Shuiping
Zhou, Weijun
author_sort Farooq, Muhammad A.
collection PubMed
description Methyl jasmonate (MJ) is an important plant growth regulator, involved in plant defense against abiotic stresses, however, its possible function in response to metal stress is poorly understood. In the present study, the effect of MJ on physiological and biochemical changes of the plants exposed to arsenic (As) stress were investigated in two Brassica napus L. cultivars (ZS 758 – a black seed type, and Zheda 622 – a yellow seed type). The As treatment at 200 μM was more phytotoxic, however, its combined application with MJ resulted in significant increase in leaf chlorophyll fluorescence, biomass production and reduced malondialdehyde content compared with As stressed plants. The application of MJ minimized the oxidative stress, as revealed via a lower level of reactive oxygen species (ROS) synthesis (H(2)O(2) and OH(-)) in leaves and the maintenance of high redox states of glutathione and ascorbate. Enhanced enzymatic activities and gene expression of important antioxidants (SOD, APX, CAT, POD), secondary metabolites (PAL, PPO, CAD) and induction of lypoxygenase gene suggest that MJ plays an effective role in the regulation of multiple transcriptional pathways which were involved in oxidative stress responses. The content of As was higher in yellow seeded plants (cv. Zheda 622) as compared to black seeded plants (ZS 758). The application of MJ significantly reduced the As content in leaves and roots of both cultivars. Findings of the present study reveal that MJ improves ROS scavenging through enhanced antioxidant defense system, secondary metabolite and reduced As contents in both the cultivars.
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spelling pubmed-48268822016-05-04 Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L. Farooq, Muhammad A. Gill, Rafaqat A. Islam, Faisal Ali, Basharat Liu, Hongbo Xu, Jianxiang He, Shuiping Zhou, Weijun Front Plant Sci Plant Science Methyl jasmonate (MJ) is an important plant growth regulator, involved in plant defense against abiotic stresses, however, its possible function in response to metal stress is poorly understood. In the present study, the effect of MJ on physiological and biochemical changes of the plants exposed to arsenic (As) stress were investigated in two Brassica napus L. cultivars (ZS 758 – a black seed type, and Zheda 622 – a yellow seed type). The As treatment at 200 μM was more phytotoxic, however, its combined application with MJ resulted in significant increase in leaf chlorophyll fluorescence, biomass production and reduced malondialdehyde content compared with As stressed plants. The application of MJ minimized the oxidative stress, as revealed via a lower level of reactive oxygen species (ROS) synthesis (H(2)O(2) and OH(-)) in leaves and the maintenance of high redox states of glutathione and ascorbate. Enhanced enzymatic activities and gene expression of important antioxidants (SOD, APX, CAT, POD), secondary metabolites (PAL, PPO, CAD) and induction of lypoxygenase gene suggest that MJ plays an effective role in the regulation of multiple transcriptional pathways which were involved in oxidative stress responses. The content of As was higher in yellow seeded plants (cv. Zheda 622) as compared to black seeded plants (ZS 758). The application of MJ significantly reduced the As content in leaves and roots of both cultivars. Findings of the present study reveal that MJ improves ROS scavenging through enhanced antioxidant defense system, secondary metabolite and reduced As contents in both the cultivars. Frontiers Media S.A. 2016-04-11 /pmc/articles/PMC4826882/ /pubmed/27148299 http://dx.doi.org/10.3389/fpls.2016.00468 Text en Copyright © 2016 Farooq, Gill, Islam, Ali, Liu, Xu, He and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Farooq, Muhammad A.
Gill, Rafaqat A.
Islam, Faisal
Ali, Basharat
Liu, Hongbo
Xu, Jianxiang
He, Shuiping
Zhou, Weijun
Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title_full Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title_fullStr Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title_full_unstemmed Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title_short Methyl Jasmonate Regulates Antioxidant Defense and Suppresses Arsenic Uptake in Brassica napus L.
title_sort methyl jasmonate regulates antioxidant defense and suppresses arsenic uptake in brassica napus l.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826882/
https://www.ncbi.nlm.nih.gov/pubmed/27148299
http://dx.doi.org/10.3389/fpls.2016.00468
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