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Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil

Bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated Hedyosmum sprucei Solms (Chloranthaceae) essential oil (EO). The antioxidant activity, performed through DPPH bioautographic assay and spectrophotometric evalu...

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Autores principales: Guerrini, Alessandra, Sacchetti, Gianni, Grandini, Alessandro, Spagnoletti, Antonella, Asanza, Mercedes, Scalvenzi, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826938/
https://www.ncbi.nlm.nih.gov/pubmed/27118979
http://dx.doi.org/10.1155/2016/1638342
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author Guerrini, Alessandra
Sacchetti, Gianni
Grandini, Alessandro
Spagnoletti, Antonella
Asanza, Mercedes
Scalvenzi, Laura
author_facet Guerrini, Alessandra
Sacchetti, Gianni
Grandini, Alessandro
Spagnoletti, Antonella
Asanza, Mercedes
Scalvenzi, Laura
author_sort Guerrini, Alessandra
collection PubMed
description Bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated Hedyosmum sprucei Solms (Chloranthaceae) essential oil (EO). The antioxidant activity, performed through DPPH bioautographic assay and spectrophotometric evaluations (IC(50) = 230 ± 10 µg/mL), seemed to be mainly due to α-cadinol and α-muurolol. (HP)TLC bioautography, focused on antimicrobial capacities, pointed out α-cadinol, α-muurolol, τ-muurolol, caryophyllene oxide, and methyleugenol as the most effective compounds against Staphylococcus aureus, considered as testing strain. Moreover, the microdilution method, assessed among a wide panel of microorganisms, revealed Listeria grayi and Staphylococcus aureus as the most sensitive among human tested strains and Clavibacter michiganensis among phytopathogens. GC-MS chemical profile showed that bioactive molecules represented only a small quantity of the whole EO: germacrene D (23.16%), β-caryophyllene (15.53%), δ-cadinene (5.50%), α-copaene (5.08%), and α-phellandrene (3.48%) were the main compounds, highlighting an uncommon composition among the genus Hedyosmum. Finally, H. sprucei EO was checked for cytotoxic potential against A549 (lung cancer) and MCF-7 (breast cancer) cell lines showing promising cytotoxic effects against both cell lines after 48 h (IC(50) A549 = 44.05 ± 2.35 µg/mL; IC(50) MCF-7 = 32.76 ± 4.92 µg/mL) and 72 h (IC(50) A549 = 43.55 ± 2.80 µg/mL; IC(50) MCF-7 = 33.64 ± 0.43 µg/mL).
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spelling pubmed-48269382016-04-26 Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil Guerrini, Alessandra Sacchetti, Gianni Grandini, Alessandro Spagnoletti, Antonella Asanza, Mercedes Scalvenzi, Laura Evid Based Complement Alternat Med Research Article Bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated Hedyosmum sprucei Solms (Chloranthaceae) essential oil (EO). The antioxidant activity, performed through DPPH bioautographic assay and spectrophotometric evaluations (IC(50) = 230 ± 10 µg/mL), seemed to be mainly due to α-cadinol and α-muurolol. (HP)TLC bioautography, focused on antimicrobial capacities, pointed out α-cadinol, α-muurolol, τ-muurolol, caryophyllene oxide, and methyleugenol as the most effective compounds against Staphylococcus aureus, considered as testing strain. Moreover, the microdilution method, assessed among a wide panel of microorganisms, revealed Listeria grayi and Staphylococcus aureus as the most sensitive among human tested strains and Clavibacter michiganensis among phytopathogens. GC-MS chemical profile showed that bioactive molecules represented only a small quantity of the whole EO: germacrene D (23.16%), β-caryophyllene (15.53%), δ-cadinene (5.50%), α-copaene (5.08%), and α-phellandrene (3.48%) were the main compounds, highlighting an uncommon composition among the genus Hedyosmum. Finally, H. sprucei EO was checked for cytotoxic potential against A549 (lung cancer) and MCF-7 (breast cancer) cell lines showing promising cytotoxic effects against both cell lines after 48 h (IC(50) A549 = 44.05 ± 2.35 µg/mL; IC(50) MCF-7 = 32.76 ± 4.92 µg/mL) and 72 h (IC(50) A549 = 43.55 ± 2.80 µg/mL; IC(50) MCF-7 = 33.64 ± 0.43 µg/mL). Hindawi Publishing Corporation 2016 2016-03-28 /pmc/articles/PMC4826938/ /pubmed/27118979 http://dx.doi.org/10.1155/2016/1638342 Text en Copyright © 2016 Alessandra Guerrini et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guerrini, Alessandra
Sacchetti, Gianni
Grandini, Alessandro
Spagnoletti, Antonella
Asanza, Mercedes
Scalvenzi, Laura
Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title_full Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title_fullStr Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title_full_unstemmed Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title_short Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil
title_sort cytotoxic effect and tlc bioautography-guided approach to detect health properties of amazonian hedyosmum sprucei essential oil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826938/
https://www.ncbi.nlm.nih.gov/pubmed/27118979
http://dx.doi.org/10.1155/2016/1638342
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