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Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells
Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC’s anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk me...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827054/ https://www.ncbi.nlm.nih.gov/pubmed/27063397 http://dx.doi.org/10.1038/srep24155 |
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author | Galler, Kerstin Requardt, Robert Pascal Glaser, Uwe Markwart, Robby Bocklitz, Thomas Bauer, Michael Popp, Jürgen Neugebauer, Ute |
author_facet | Galler, Kerstin Requardt, Robert Pascal Glaser, Uwe Markwart, Robby Bocklitz, Thomas Bauer, Michael Popp, Jürgen Neugebauer, Ute |
author_sort | Galler, Kerstin |
collection | PubMed |
description | Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC’s anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive wavelength 785 nm is introduced for the assessment of the retinoid state of single HSCs in freshly isolated, unprocessed murine liver lobes. A quantitative estimation of the cellular retinoid content is derived. Implications of the retinoid content on hepatic health state are reported. The Raman-based results are integrated with histological assessments of the tissue samples. This spectroscopic approach enables single cell analysis regarding an important cellular feature in unharmed tissue. |
format | Online Article Text |
id | pubmed-4827054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48270542016-04-19 Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells Galler, Kerstin Requardt, Robert Pascal Glaser, Uwe Markwart, Robby Bocklitz, Thomas Bauer, Michael Popp, Jürgen Neugebauer, Ute Sci Rep Article Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC’s anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive wavelength 785 nm is introduced for the assessment of the retinoid state of single HSCs in freshly isolated, unprocessed murine liver lobes. A quantitative estimation of the cellular retinoid content is derived. Implications of the retinoid content on hepatic health state are reported. The Raman-based results are integrated with histological assessments of the tissue samples. This spectroscopic approach enables single cell analysis regarding an important cellular feature in unharmed tissue. Nature Publishing Group 2016-04-11 /pmc/articles/PMC4827054/ /pubmed/27063397 http://dx.doi.org/10.1038/srep24155 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Galler, Kerstin Requardt, Robert Pascal Glaser, Uwe Markwart, Robby Bocklitz, Thomas Bauer, Michael Popp, Jürgen Neugebauer, Ute Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title | Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title_full | Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title_fullStr | Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title_full_unstemmed | Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title_short | Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells |
title_sort | single cell analysis in native tissue: quantification of the retinoid content of hepatic stellate cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827054/ https://www.ncbi.nlm.nih.gov/pubmed/27063397 http://dx.doi.org/10.1038/srep24155 |
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