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The Brain Metabolome of Male Rats across the Lifespan
Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. To...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827083/ https://www.ncbi.nlm.nih.gov/pubmed/27063670 http://dx.doi.org/10.1038/srep24125 |
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author | Zheng, Xiaojiao Chen, Tianlu Zhao, Aihua Wang, Xiaoyan Xie, Guoxiang Huang, Fengjie Liu, Jiajian Zhao, Qing Wang, Shouli Wang, Chongchong Zhou, Mingmei Panee, Jun He, Zhigang Jia, Wei |
author_facet | Zheng, Xiaojiao Chen, Tianlu Zhao, Aihua Wang, Xiaoyan Xie, Guoxiang Huang, Fengjie Liu, Jiajian Zhao, Qing Wang, Shouli Wang, Chongchong Zhou, Mingmei Panee, Jun He, Zhigang Jia, Wei |
author_sort | Zheng, Xiaojiao |
collection | PubMed |
description | Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolites were identified and 232 of them were quantitated. Compared with anatomical regions, age had a greater effect on variations in the brain metabolome. Lipids, fatty acids and amino acids accounted for the largest proportions of the brain metabolome, and their concentrations varied across the lifespan. The levels of polyunsaturated fatty acids were higher in infancy (week 1 to week 3) compared with later ages, and the ratio of omega-6 to omega-3 fatty acids increased in the aged brain (week 56 to week 111). Importantly, a panel of 20 bile acids were quantitatively measured, most of which have not previously been documented in the brain metabolome. This study extends the breadth of the mammalian brain metabolome as well as our knowledge of functional brain development, both of which are critically important to move the brain science forward. |
format | Online Article Text |
id | pubmed-4827083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48270832016-04-19 The Brain Metabolome of Male Rats across the Lifespan Zheng, Xiaojiao Chen, Tianlu Zhao, Aihua Wang, Xiaoyan Xie, Guoxiang Huang, Fengjie Liu, Jiajian Zhao, Qing Wang, Shouli Wang, Chongchong Zhou, Mingmei Panee, Jun He, Zhigang Jia, Wei Sci Rep Article Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolites were identified and 232 of them were quantitated. Compared with anatomical regions, age had a greater effect on variations in the brain metabolome. Lipids, fatty acids and amino acids accounted for the largest proportions of the brain metabolome, and their concentrations varied across the lifespan. The levels of polyunsaturated fatty acids were higher in infancy (week 1 to week 3) compared with later ages, and the ratio of omega-6 to omega-3 fatty acids increased in the aged brain (week 56 to week 111). Importantly, a panel of 20 bile acids were quantitatively measured, most of which have not previously been documented in the brain metabolome. This study extends the breadth of the mammalian brain metabolome as well as our knowledge of functional brain development, both of which are critically important to move the brain science forward. Nature Publishing Group 2016-04-11 /pmc/articles/PMC4827083/ /pubmed/27063670 http://dx.doi.org/10.1038/srep24125 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zheng, Xiaojiao Chen, Tianlu Zhao, Aihua Wang, Xiaoyan Xie, Guoxiang Huang, Fengjie Liu, Jiajian Zhao, Qing Wang, Shouli Wang, Chongchong Zhou, Mingmei Panee, Jun He, Zhigang Jia, Wei The Brain Metabolome of Male Rats across the Lifespan |
title | The Brain Metabolome of Male Rats across the Lifespan |
title_full | The Brain Metabolome of Male Rats across the Lifespan |
title_fullStr | The Brain Metabolome of Male Rats across the Lifespan |
title_full_unstemmed | The Brain Metabolome of Male Rats across the Lifespan |
title_short | The Brain Metabolome of Male Rats across the Lifespan |
title_sort | brain metabolome of male rats across the lifespan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827083/ https://www.ncbi.nlm.nih.gov/pubmed/27063670 http://dx.doi.org/10.1038/srep24125 |
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