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The Brain Metabolome of Male Rats across the Lifespan

Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. To...

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Autores principales: Zheng, Xiaojiao, Chen, Tianlu, Zhao, Aihua, Wang, Xiaoyan, Xie, Guoxiang, Huang, Fengjie, Liu, Jiajian, Zhao, Qing, Wang, Shouli, Wang, Chongchong, Zhou, Mingmei, Panee, Jun, He, Zhigang, Jia, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827083/
https://www.ncbi.nlm.nih.gov/pubmed/27063670
http://dx.doi.org/10.1038/srep24125
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author Zheng, Xiaojiao
Chen, Tianlu
Zhao, Aihua
Wang, Xiaoyan
Xie, Guoxiang
Huang, Fengjie
Liu, Jiajian
Zhao, Qing
Wang, Shouli
Wang, Chongchong
Zhou, Mingmei
Panee, Jun
He, Zhigang
Jia, Wei
author_facet Zheng, Xiaojiao
Chen, Tianlu
Zhao, Aihua
Wang, Xiaoyan
Xie, Guoxiang
Huang, Fengjie
Liu, Jiajian
Zhao, Qing
Wang, Shouli
Wang, Chongchong
Zhou, Mingmei
Panee, Jun
He, Zhigang
Jia, Wei
author_sort Zheng, Xiaojiao
collection PubMed
description Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolites were identified and 232 of them were quantitated. Compared with anatomical regions, age had a greater effect on variations in the brain metabolome. Lipids, fatty acids and amino acids accounted for the largest proportions of the brain metabolome, and their concentrations varied across the lifespan. The levels of polyunsaturated fatty acids were higher in infancy (week 1 to week 3) compared with later ages, and the ratio of omega-6 to omega-3 fatty acids increased in the aged brain (week 56 to week 111). Importantly, a panel of 20 bile acids were quantitatively measured, most of which have not previously been documented in the brain metabolome. This study extends the breadth of the mammalian brain metabolome as well as our knowledge of functional brain development, both of which are critically important to move the brain science forward.
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spelling pubmed-48270832016-04-19 The Brain Metabolome of Male Rats across the Lifespan Zheng, Xiaojiao Chen, Tianlu Zhao, Aihua Wang, Xiaoyan Xie, Guoxiang Huang, Fengjie Liu, Jiajian Zhao, Qing Wang, Shouli Wang, Chongchong Zhou, Mingmei Panee, Jun He, Zhigang Jia, Wei Sci Rep Article Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolites were identified and 232 of them were quantitated. Compared with anatomical regions, age had a greater effect on variations in the brain metabolome. Lipids, fatty acids and amino acids accounted for the largest proportions of the brain metabolome, and their concentrations varied across the lifespan. The levels of polyunsaturated fatty acids were higher in infancy (week 1 to week 3) compared with later ages, and the ratio of omega-6 to omega-3 fatty acids increased in the aged brain (week 56 to week 111). Importantly, a panel of 20 bile acids were quantitatively measured, most of which have not previously been documented in the brain metabolome. This study extends the breadth of the mammalian brain metabolome as well as our knowledge of functional brain development, both of which are critically important to move the brain science forward. Nature Publishing Group 2016-04-11 /pmc/articles/PMC4827083/ /pubmed/27063670 http://dx.doi.org/10.1038/srep24125 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zheng, Xiaojiao
Chen, Tianlu
Zhao, Aihua
Wang, Xiaoyan
Xie, Guoxiang
Huang, Fengjie
Liu, Jiajian
Zhao, Qing
Wang, Shouli
Wang, Chongchong
Zhou, Mingmei
Panee, Jun
He, Zhigang
Jia, Wei
The Brain Metabolome of Male Rats across the Lifespan
title The Brain Metabolome of Male Rats across the Lifespan
title_full The Brain Metabolome of Male Rats across the Lifespan
title_fullStr The Brain Metabolome of Male Rats across the Lifespan
title_full_unstemmed The Brain Metabolome of Male Rats across the Lifespan
title_short The Brain Metabolome of Male Rats across the Lifespan
title_sort brain metabolome of male rats across the lifespan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827083/
https://www.ncbi.nlm.nih.gov/pubmed/27063670
http://dx.doi.org/10.1038/srep24125
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