Accurate quantification of supercoiled DNA by digital PCR

Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without the help of standards. However, it can generate false results when the PCR conditions are not optimized. A recent international comparison (CCQM P154) showed that most laboratories...

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Detalles Bibliográficos
Autores principales: Dong, Lianhua, Yoo, Hee-Bong, Wang, Jing, Park, Sang-Ryoul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827127/
https://www.ncbi.nlm.nih.gov/pubmed/27063649
http://dx.doi.org/10.1038/srep24230

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827127/
https://www.ncbi.nlm.nih.gov/pubmed/27063649
http://dx.doi.org/10.1038/srep24230

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