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Accurate quantification of supercoiled DNA by digital PCR
Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without the help of standards. However, it can generate false results when the PCR conditions are not optimized. A recent international comparison (CCQM P154) showed that most laboratories...
Autores principales: | Dong, Lianhua, Yoo, Hee-Bong, Wang, Jing, Park, Sang-Ryoul |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827127/ https://www.ncbi.nlm.nih.gov/pubmed/27063649 http://dx.doi.org/10.1038/srep24230 |
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