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Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates
BACKGROUND: Accurate adjustment for the amplification efficiency (AE) is an important part of real-time quantitative polymerase chain reaction (qPCR) experiments. The most commonly used correction strategy is to estimate the AE by dilution experiments and use this as a plug-in when efficiency correc...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827196/ https://www.ncbi.nlm.nih.gov/pubmed/27067838 http://dx.doi.org/10.1186/s12859-016-0997-6 |