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Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract

Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that take important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Natural products are on the rise for their potential to provide r...

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Autores principales: Bae, Min-Joo, Karadeniz, Fatih, Lee, Seul-Gi, Seo, Youngwan, Kong, Chang-Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Food Science and Nutrition 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827633/
https://www.ncbi.nlm.nih.gov/pubmed/27069904
http://dx.doi.org/10.3746/pnf.2016.21.1.38
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author Bae, Min-Joo
Karadeniz, Fatih
Lee, Seul-Gi
Seo, Youngwan
Kong, Chang-Suk
author_facet Bae, Min-Joo
Karadeniz, Fatih
Lee, Seul-Gi
Seo, Youngwan
Kong, Chang-Suk
author_sort Bae, Min-Joo
collection PubMed
description Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that take important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Natural products are on the rise for their potential to provide remarkable health benefits. In this context, halophytes have been of interest in the nutraceutical field with reported instances of isolation of bioactive compounds. In this study, Limonium tetragonum, an edible halophyte, was studied for its ability to inhibit MMP-2 and -9 using HT1080 fibrosarcoma cells. Results showed that L. tetragonum extract was able to inhibit the enzymatic activity and mRNA expression of MMP-2 and -9 according to gelatin zymography and RT-PCR assays, respectively, but it was not able to significantly change the MMP pathway related factors such as tissue inhibitors of metalloproteinases. Also, Mitogen-activated protein kinases pathway-related protein levels and their phosphorylation were assayed. While the phosphorylated p38 levels were decreased, extracellular signal-regulated kinase and c-Jun N-terminal kinase were not affected by L. tetragonum treatment. In conclusion, it was suggested that L. tetragonum contains substances acting as MMP inhibitors on enzymatic activity rather than intracellular pathway intervention, which could be useful for further utilization of L. tetragonum as a source for anti-MMP agents.
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spelling pubmed-48276332016-04-11 Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract Bae, Min-Joo Karadeniz, Fatih Lee, Seul-Gi Seo, Youngwan Kong, Chang-Suk Prev Nutr Food Sci Articles Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that take important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Natural products are on the rise for their potential to provide remarkable health benefits. In this context, halophytes have been of interest in the nutraceutical field with reported instances of isolation of bioactive compounds. In this study, Limonium tetragonum, an edible halophyte, was studied for its ability to inhibit MMP-2 and -9 using HT1080 fibrosarcoma cells. Results showed that L. tetragonum extract was able to inhibit the enzymatic activity and mRNA expression of MMP-2 and -9 according to gelatin zymography and RT-PCR assays, respectively, but it was not able to significantly change the MMP pathway related factors such as tissue inhibitors of metalloproteinases. Also, Mitogen-activated protein kinases pathway-related protein levels and their phosphorylation were assayed. While the phosphorylated p38 levels were decreased, extracellular signal-regulated kinase and c-Jun N-terminal kinase were not affected by L. tetragonum treatment. In conclusion, it was suggested that L. tetragonum contains substances acting as MMP inhibitors on enzymatic activity rather than intracellular pathway intervention, which could be useful for further utilization of L. tetragonum as a source for anti-MMP agents. The Korean Society of Food Science and Nutrition 2016-03 2016-03-31 /pmc/articles/PMC4827633/ /pubmed/27069904 http://dx.doi.org/10.3746/pnf.2016.21.1.38 Text en Copyright © 2016 by The Korean Society of Food Science and Nutrition This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Bae, Min-Joo
Karadeniz, Fatih
Lee, Seul-Gi
Seo, Youngwan
Kong, Chang-Suk
Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title_full Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title_fullStr Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title_full_unstemmed Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title_short Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
title_sort inhibition of mmp-2 and mmp-9 activities by limonium tetragonum extract
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827633/
https://www.ncbi.nlm.nih.gov/pubmed/27069904
http://dx.doi.org/10.3746/pnf.2016.21.1.38
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