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Real-time PCR probe optimization using design of experiments approach

Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization. In this study, we present the use of statistical design of experiments (DOE) approach as a general guideline for probe optimization and more specifically focus...

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Detalles Bibliográficos
Autores principales:	Wadle, S., Lehnert, M., Rubenwolf, S., Zengerle, R., von Stetten, F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827641/ https://www.ncbi.nlm.nih.gov/pubmed/27077046 http://dx.doi.org/10.1016/j.bdq.2015.12.002

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