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Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)

Somatic embryogenesis has been shown to be an efficient tool for studying processes based on cell growth and development. The fine regulation of the cell cycle is essential for proper embryo formation during the process of somatic embryogenesis. The aims of the present work were to identify and perf...

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Autores principales: Douétts-Peres, Jackellinne C., Cruz, Marco Antônio L., Reis, Ricardo S., Heringer, Angelo S., de Oliveira, Eduardo A. G., Elbl, Paula M., Floh, Eny I. S., Silveira, Vanildo, Santa-Catarina, Claudete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827878/
https://www.ncbi.nlm.nih.gov/pubmed/27064899
http://dx.doi.org/10.1371/journal.pone.0153528
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author Douétts-Peres, Jackellinne C.
Cruz, Marco Antônio L.
Reis, Ricardo S.
Heringer, Angelo S.
de Oliveira, Eduardo A. G.
Elbl, Paula M.
Floh, Eny I. S.
Silveira, Vanildo
Santa-Catarina, Claudete
author_facet Douétts-Peres, Jackellinne C.
Cruz, Marco Antônio L.
Reis, Ricardo S.
Heringer, Angelo S.
de Oliveira, Eduardo A. G.
Elbl, Paula M.
Floh, Eny I. S.
Silveira, Vanildo
Santa-Catarina, Claudete
author_sort Douétts-Peres, Jackellinne C.
collection PubMed
description Somatic embryogenesis has been shown to be an efficient tool for studying processes based on cell growth and development. The fine regulation of the cell cycle is essential for proper embryo formation during the process of somatic embryogenesis. The aims of the present work were to identify and perform a structural and functional characterization of Mps1 and to analyze the effects of the inhibition of this protein on cellular growth and pro-embryogenic mass (PEM) morphology in embryogenic cultures of A. angustifolia. A single-copy Mps1 gene named AaMps1 was retrieved from the A. angustifolia transcriptome database, and through a mass spectrometry approach, AaMps1 was identified and quantified in embryogenic cultures. The Mps1 inhibitor SP600125 (10 μM) inhibited cellular growth and changed PEMs, and these effects were accompanied by a reduction in AaMps1 protein levels in embryogenic cultures. Our work has identified the Mps1 protein in a gymnosperm species for the first time, and we have shown that inhibiting Mps1 affects cellular growth and PEM differentiation during A. angustifolia somatic embryogenesis. These data will be useful for better understanding cell cycle control during somatic embryogenesis in plants.
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spelling pubmed-48278782016-04-22 Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae) Douétts-Peres, Jackellinne C. Cruz, Marco Antônio L. Reis, Ricardo S. Heringer, Angelo S. de Oliveira, Eduardo A. G. Elbl, Paula M. Floh, Eny I. S. Silveira, Vanildo Santa-Catarina, Claudete PLoS One Research Article Somatic embryogenesis has been shown to be an efficient tool for studying processes based on cell growth and development. The fine regulation of the cell cycle is essential for proper embryo formation during the process of somatic embryogenesis. The aims of the present work were to identify and perform a structural and functional characterization of Mps1 and to analyze the effects of the inhibition of this protein on cellular growth and pro-embryogenic mass (PEM) morphology in embryogenic cultures of A. angustifolia. A single-copy Mps1 gene named AaMps1 was retrieved from the A. angustifolia transcriptome database, and through a mass spectrometry approach, AaMps1 was identified and quantified in embryogenic cultures. The Mps1 inhibitor SP600125 (10 μM) inhibited cellular growth and changed PEMs, and these effects were accompanied by a reduction in AaMps1 protein levels in embryogenic cultures. Our work has identified the Mps1 protein in a gymnosperm species for the first time, and we have shown that inhibiting Mps1 affects cellular growth and PEM differentiation during A. angustifolia somatic embryogenesis. These data will be useful for better understanding cell cycle control during somatic embryogenesis in plants. Public Library of Science 2016-04-11 /pmc/articles/PMC4827878/ /pubmed/27064899 http://dx.doi.org/10.1371/journal.pone.0153528 Text en © 2016 Douétts-Peres et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Douétts-Peres, Jackellinne C.
Cruz, Marco Antônio L.
Reis, Ricardo S.
Heringer, Angelo S.
de Oliveira, Eduardo A. G.
Elbl, Paula M.
Floh, Eny I. S.
Silveira, Vanildo
Santa-Catarina, Claudete
Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title_full Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title_fullStr Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title_full_unstemmed Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title_short Mps1 (Monopolar Spindle 1) Protein Inhibition Affects Cellular Growth and Pro-Embryogenic Masses Morphology in Embryogenic Cultures of Araucaria angustifolia (Araucariaceae)
title_sort mps1 (monopolar spindle 1) protein inhibition affects cellular growth and pro-embryogenic masses morphology in embryogenic cultures of araucaria angustifolia (araucariaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827878/
https://www.ncbi.nlm.nih.gov/pubmed/27064899
http://dx.doi.org/10.1371/journal.pone.0153528
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