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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

One of the major questions in microbial ecology is “who is there?” This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally,...

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Detalles Bibliográficos
Autores principales: Sanschagrin, Sylvie, Yergeau, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828026/
https://www.ncbi.nlm.nih.gov/pubmed/25226019
http://dx.doi.org/10.3791/51709