Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose a valuable tool to understand the underlying mechanisms of these diseases. We therefore present a straightforward protocol for the isolation of human monocytes from buffy c...

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Detalles Bibliográficos
Autores principales: Menck, Kerstin, Behme, Daniel, Pantke, Mathias, Reiling, Norbert, Binder, Claudia, Pukrop, Tobias, Klemm, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828059/
https://www.ncbi.nlm.nih.gov/pubmed/25226391
http://dx.doi.org/10.3791/51554

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828059/
https://www.ncbi.nlm.nih.gov/pubmed/25226391
http://dx.doi.org/10.3791/51554

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