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Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton
Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale con...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828694/ https://www.ncbi.nlm.nih.gov/pubmed/27044896 http://dx.doi.org/10.1083/jcb.201512029 |
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author | Bridges, Andrew A. Jentzsch, Maximilian S. Oakes, Patrick W. Occhipinti, Patricia Gladfelter, Amy S. |
author_facet | Bridges, Andrew A. Jentzsch, Maximilian S. Oakes, Patrick W. Occhipinti, Patricia Gladfelter, Amy S. |
author_sort | Bridges, Andrew A. |
collection | PubMed |
description | Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape. |
format | Online Article Text |
id | pubmed-4828694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48286942016-10-11 Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton Bridges, Andrew A. Jentzsch, Maximilian S. Oakes, Patrick W. Occhipinti, Patricia Gladfelter, Amy S. J Cell Biol Research Articles Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape. The Rockefeller University Press 2016-04-11 /pmc/articles/PMC4828694/ /pubmed/27044896 http://dx.doi.org/10.1083/jcb.201512029 Text en © 2016 Bridges et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Bridges, Andrew A. Jentzsch, Maximilian S. Oakes, Patrick W. Occhipinti, Patricia Gladfelter, Amy S. Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title | Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title_full | Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title_fullStr | Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title_full_unstemmed | Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title_short | Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
title_sort | micron-scale plasma membrane curvature is recognized by the septin cytoskeleton |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828694/ https://www.ncbi.nlm.nih.gov/pubmed/27044896 http://dx.doi.org/10.1083/jcb.201512029 |
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