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The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo

BACKGROUND: The deleterious effects of dietary trans fatty acids (tFAs) on human health are well documented. Although significantly reduced or banned in various countries, tFAs may trigger long-term responses that would represent a valid human health concern, particularly if tFAs alter the epigenome...

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Autores principales: Flores-Sierra, José, Arredondo-Guerrero, Martín, Cervantes-Paz, Braulio, Rodríguez-Ríos, Dalia, Alvarado-Caudillo, Yolanda, Nielsen, Finn C., Wrobel, Katarzyna, Wrobel, Kazimierz, Zaina, Silvio, Lund, Gertrud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828757/
https://www.ncbi.nlm.nih.gov/pubmed/27068706
http://dx.doi.org/10.1186/s12944-016-0243-2
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author Flores-Sierra, José
Arredondo-Guerrero, Martín
Cervantes-Paz, Braulio
Rodríguez-Ríos, Dalia
Alvarado-Caudillo, Yolanda
Nielsen, Finn C.
Wrobel, Katarzyna
Wrobel, Kazimierz
Zaina, Silvio
Lund, Gertrud
author_facet Flores-Sierra, José
Arredondo-Guerrero, Martín
Cervantes-Paz, Braulio
Rodríguez-Ríos, Dalia
Alvarado-Caudillo, Yolanda
Nielsen, Finn C.
Wrobel, Katarzyna
Wrobel, Kazimierz
Zaina, Silvio
Lund, Gertrud
author_sort Flores-Sierra, José
collection PubMed
description BACKGROUND: The deleterious effects of dietary trans fatty acids (tFAs) on human health are well documented. Although significantly reduced or banned in various countries, tFAs may trigger long-term responses that would represent a valid human health concern, particularly if tFAs alter the epigenome. METHODS: Based on these considerations, we asked whether the tFA elaidic acid (EA; tC18:1) has any effects on global DNA methylation and the transcriptome in cultured human THP-1 monocytes, and whether the progeny of EA-supplemented dams during either pregnancy or lactation in mice (n = 20 per group) show any epigenetic change after exposure. RESULTS: EA induced a biphasic effect on global DNA methylation in THP-1 cells, i.e. hypermethylation in the 1–50 μM concentration range, followed by hypomethylation up to the 200 μM dose. On the other hand, the cis isomer oleic acid (OA), a fatty acid with documented beneficial effects on human health, exerted a distinct response, i.e. its effects were weaker and only partially overlapping with EA’s. The maximal differential response between EA and OA was observed at the 50 μM dose. Array expression data revealed that EA induced a pro-inflammatory and adipogenic transcriptional profile compared with OA, although with modest effects on selected (n = 9) gene promoter methylation. In mice, maternal EA supplementation in utero or via the breastmilk induced global adipose tissue DNA hypermethylation in the progeny, that was detectable postnatally at the age of 3 months. CONCLUSION: We document that global DNA hypermethylation is a specific and consistent response to EA in cell culture and in mice, and that EA may exert long-term effects on the epigenome following maternal exposure. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0243-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-48287572016-04-13 The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo Flores-Sierra, José Arredondo-Guerrero, Martín Cervantes-Paz, Braulio Rodríguez-Ríos, Dalia Alvarado-Caudillo, Yolanda Nielsen, Finn C. Wrobel, Katarzyna Wrobel, Kazimierz Zaina, Silvio Lund, Gertrud Lipids Health Dis Research BACKGROUND: The deleterious effects of dietary trans fatty acids (tFAs) on human health are well documented. Although significantly reduced or banned in various countries, tFAs may trigger long-term responses that would represent a valid human health concern, particularly if tFAs alter the epigenome. METHODS: Based on these considerations, we asked whether the tFA elaidic acid (EA; tC18:1) has any effects on global DNA methylation and the transcriptome in cultured human THP-1 monocytes, and whether the progeny of EA-supplemented dams during either pregnancy or lactation in mice (n = 20 per group) show any epigenetic change after exposure. RESULTS: EA induced a biphasic effect on global DNA methylation in THP-1 cells, i.e. hypermethylation in the 1–50 μM concentration range, followed by hypomethylation up to the 200 μM dose. On the other hand, the cis isomer oleic acid (OA), a fatty acid with documented beneficial effects on human health, exerted a distinct response, i.e. its effects were weaker and only partially overlapping with EA’s. The maximal differential response between EA and OA was observed at the 50 μM dose. Array expression data revealed that EA induced a pro-inflammatory and adipogenic transcriptional profile compared with OA, although with modest effects on selected (n = 9) gene promoter methylation. In mice, maternal EA supplementation in utero or via the breastmilk induced global adipose tissue DNA hypermethylation in the progeny, that was detectable postnatally at the age of 3 months. CONCLUSION: We document that global DNA hypermethylation is a specific and consistent response to EA in cell culture and in mice, and that EA may exert long-term effects on the epigenome following maternal exposure. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0243-2) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-12 /pmc/articles/PMC4828757/ /pubmed/27068706 http://dx.doi.org/10.1186/s12944-016-0243-2 Text en © Flores-Sierra et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Flores-Sierra, José
Arredondo-Guerrero, Martín
Cervantes-Paz, Braulio
Rodríguez-Ríos, Dalia
Alvarado-Caudillo, Yolanda
Nielsen, Finn C.
Wrobel, Katarzyna
Wrobel, Kazimierz
Zaina, Silvio
Lund, Gertrud
The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title_full The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title_fullStr The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title_full_unstemmed The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title_short The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo
title_sort trans fatty acid elaidate affects the global dna methylation profile of cultured cells and in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828757/
https://www.ncbi.nlm.nih.gov/pubmed/27068706
http://dx.doi.org/10.1186/s12944-016-0243-2
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