Cargando…

Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode

Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak po...

Descripción completa

Detalles Bibliográficos
Autores principales: Nikodimos, Yosef, Amare, Meareg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828882/
https://www.ncbi.nlm.nih.gov/pubmed/27119041
http://dx.doi.org/10.1155/2016/3612943
_version_ 1782426671299690496
author Nikodimos, Yosef
Amare, Meareg
author_facet Nikodimos, Yosef
Amare, Meareg
author_sort Nikodimos, Yosef
collection PubMed
description Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10(−6) to 5.0 × 10(−4) M with a correlation coefficient, method detection limit (based on s = 3σ), and limit of quantification of 0.999, 2.97 × 10(−7) M and 9.91 × 10(−7) M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets.
format Online
Article
Text
id pubmed-4828882
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-48288822016-04-26 Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode Nikodimos, Yosef Amare, Meareg J Anal Methods Chem Research Article Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10(−6) to 5.0 × 10(−4) M with a correlation coefficient, method detection limit (based on s = 3σ), and limit of quantification of 0.999, 2.97 × 10(−7) M and 9.91 × 10(−7) M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets. Hindawi Publishing Corporation 2016 2016-03-29 /pmc/articles/PMC4828882/ /pubmed/27119041 http://dx.doi.org/10.1155/2016/3612943 Text en Copyright © 2016 Y. Nikodimos and M. Amare. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nikodimos, Yosef
Amare, Meareg
Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_full Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_fullStr Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_full_unstemmed Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_short Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_sort electrochemical determination of metronidazole in tablet samples using carbon paste electrode
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828882/
https://www.ncbi.nlm.nih.gov/pubmed/27119041
http://dx.doi.org/10.1155/2016/3612943
work_keys_str_mv AT nikodimosyosef electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode
AT amaremeareg electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode