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Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak po...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828882/ https://www.ncbi.nlm.nih.gov/pubmed/27119041 http://dx.doi.org/10.1155/2016/3612943 |
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author | Nikodimos, Yosef Amare, Meareg |
author_facet | Nikodimos, Yosef Amare, Meareg |
author_sort | Nikodimos, Yosef |
collection | PubMed |
description | Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10(−6) to 5.0 × 10(−4) M with a correlation coefficient, method detection limit (based on s = 3σ), and limit of quantification of 0.999, 2.97 × 10(−7) M and 9.91 × 10(−7) M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets. |
format | Online Article Text |
id | pubmed-4828882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48288822016-04-26 Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode Nikodimos, Yosef Amare, Meareg J Anal Methods Chem Research Article Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10(−6) to 5.0 × 10(−4) M with a correlation coefficient, method detection limit (based on s = 3σ), and limit of quantification of 0.999, 2.97 × 10(−7) M and 9.91 × 10(−7) M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets. Hindawi Publishing Corporation 2016 2016-03-29 /pmc/articles/PMC4828882/ /pubmed/27119041 http://dx.doi.org/10.1155/2016/3612943 Text en Copyright © 2016 Y. Nikodimos and M. Amare. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nikodimos, Yosef Amare, Meareg Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title | Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title_full | Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title_fullStr | Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title_full_unstemmed | Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title_short | Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode |
title_sort | electrochemical determination of metronidazole in tablet samples using carbon paste electrode |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828882/ https://www.ncbi.nlm.nih.gov/pubmed/27119041 http://dx.doi.org/10.1155/2016/3612943 |
work_keys_str_mv | AT nikodimosyosef electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode AT amaremeareg electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode |