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Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts
Emerging evidence suggests Jumonji domain-containing proteins are epigenetic regulators in diverse biological processes including cellular differentiation and proliferation. RNA interference-based analyses combined with gene expression profiling can effectively characterize the cellular functions of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828940/ https://www.ncbi.nlm.nih.gov/pubmed/27071056 http://dx.doi.org/10.1038/sdata.2016.22 |
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author | Hu, Yu-Jie Imbalzano, Anthony N. |
author_facet | Hu, Yu-Jie Imbalzano, Anthony N. |
author_sort | Hu, Yu-Jie |
collection | PubMed |
description | Emerging evidence suggests Jumonji domain-containing proteins are epigenetic regulators in diverse biological processes including cellular differentiation and proliferation. RNA interference-based analyses combined with gene expression profiling can effectively characterize the cellular functions of these enzymes. We found that the depletion of Jumonji domain-containing protein 6 (JMJD6) and its paralog protein Jumonji domain-containing protein 4 (JMJD4) individually by small hairpin RNAs (shRNAs) slowed cell proliferation of mouse NIH3T3 fibroblasts. We subsequently performed gene expression profiling on both JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts using the Affymetrix GeneChip Mouse Exon 1.0 ST Array. Here we report the gene profiling datasets along with the experimental procedures. The information can be used to further investigate how JMJD6 and JMJD4 affect gene expression and cellular physiology. |
format | Online Article Text |
id | pubmed-4828940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48289402016-04-28 Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts Hu, Yu-Jie Imbalzano, Anthony N. Sci Data Data Descriptor Emerging evidence suggests Jumonji domain-containing proteins are epigenetic regulators in diverse biological processes including cellular differentiation and proliferation. RNA interference-based analyses combined with gene expression profiling can effectively characterize the cellular functions of these enzymes. We found that the depletion of Jumonji domain-containing protein 6 (JMJD6) and its paralog protein Jumonji domain-containing protein 4 (JMJD4) individually by small hairpin RNAs (shRNAs) slowed cell proliferation of mouse NIH3T3 fibroblasts. We subsequently performed gene expression profiling on both JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts using the Affymetrix GeneChip Mouse Exon 1.0 ST Array. Here we report the gene profiling datasets along with the experimental procedures. The information can be used to further investigate how JMJD6 and JMJD4 affect gene expression and cellular physiology. Nature Publishing Group 2016-04-12 /pmc/articles/PMC4828940/ /pubmed/27071056 http://dx.doi.org/10.1038/sdata.2016.22 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse. |
spellingShingle | Data Descriptor Hu, Yu-Jie Imbalzano, Anthony N. Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title | Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title_full | Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title_fullStr | Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title_full_unstemmed | Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title_short | Global gene expression profiling of JMJD6- and JMJD4-depleted mouse NIH3T3 fibroblasts |
title_sort | global gene expression profiling of jmjd6- and jmjd4-depleted mouse nih3t3 fibroblasts |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828940/ https://www.ncbi.nlm.nih.gov/pubmed/27071056 http://dx.doi.org/10.1038/sdata.2016.22 |
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