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Verapamil inhibits scar formation after peripheral nerve repair in vivo
The calcium channel blocker, verapamil, has been shown to reduce scar formation by inhibiting fibroblast adhesion and proliferation in vitro. It was not clear whether topical application of verapamil after surgical repair of the nerve in vivo could inhibit the formation of excessive scar tissue. In...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829020/ https://www.ncbi.nlm.nih.gov/pubmed/27127494 http://dx.doi.org/10.4103/1673-5374.179075 |
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author | Han, A-chao Deng, Jing-xiu Huang, Qi-shun Zheng, Huai-yuan Zhou, Pan Liu, Zhi-wei Chen, Zhen-bing |
author_facet | Han, A-chao Deng, Jing-xiu Huang, Qi-shun Zheng, Huai-yuan Zhou, Pan Liu, Zhi-wei Chen, Zhen-bing |
author_sort | Han, A-chao |
collection | PubMed |
description | The calcium channel blocker, verapamil, has been shown to reduce scar formation by inhibiting fibroblast adhesion and proliferation in vitro. It was not clear whether topical application of verapamil after surgical repair of the nerve in vivo could inhibit the formation of excessive scar tissue. In this study, the right sciatic nerve of adult Sprague-Dawley rats was transected and sutured with No. 10-0 suture. The stoma was wrapped with gelfoam soaked with verapamil solution for 4 weeks. Compared with the control group (stoma wrapped with gelfoam soaked with physiological saline), the verapamil application inhibited the secretion of extracellular matrix from fibroblasts in vivo, suppressed type I and III collagen secretion and increased the total number of axons and the number of myelinated axons. These findings suggest that verapamil could reduce the formation of scar tissue and promote axon growth after peripheral nerve repair. |
format | Online Article Text |
id | pubmed-4829020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-48290202016-04-28 Verapamil inhibits scar formation after peripheral nerve repair in vivo Han, A-chao Deng, Jing-xiu Huang, Qi-shun Zheng, Huai-yuan Zhou, Pan Liu, Zhi-wei Chen, Zhen-bing Neural Regen Res Research Article The calcium channel blocker, verapamil, has been shown to reduce scar formation by inhibiting fibroblast adhesion and proliferation in vitro. It was not clear whether topical application of verapamil after surgical repair of the nerve in vivo could inhibit the formation of excessive scar tissue. In this study, the right sciatic nerve of adult Sprague-Dawley rats was transected and sutured with No. 10-0 suture. The stoma was wrapped with gelfoam soaked with verapamil solution for 4 weeks. Compared with the control group (stoma wrapped with gelfoam soaked with physiological saline), the verapamil application inhibited the secretion of extracellular matrix from fibroblasts in vivo, suppressed type I and III collagen secretion and increased the total number of axons and the number of myelinated axons. These findings suggest that verapamil could reduce the formation of scar tissue and promote axon growth after peripheral nerve repair. Medknow Publications & Media Pvt Ltd 2016-03 /pmc/articles/PMC4829020/ /pubmed/27127494 http://dx.doi.org/10.4103/1673-5374.179075 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Han, A-chao Deng, Jing-xiu Huang, Qi-shun Zheng, Huai-yuan Zhou, Pan Liu, Zhi-wei Chen, Zhen-bing Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title | Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title_full | Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title_fullStr | Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title_full_unstemmed | Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title_short | Verapamil inhibits scar formation after peripheral nerve repair in vivo |
title_sort | verapamil inhibits scar formation after peripheral nerve repair in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829020/ https://www.ncbi.nlm.nih.gov/pubmed/27127494 http://dx.doi.org/10.4103/1673-5374.179075 |
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