Cargando…

Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?

BACKGROUND: It is unknown whether Whole-Body Hypothermia (WBH) affects pulmonary function. In vitro studies, at relatively low temperatures, suggest that hypothermia may induce significant changes to the surfactant composition. The effect of WBH on surfactant kinetics in newborn infants is unknown....

Descripción completa

Detalles Bibliográficos
Autores principales: Nespeca, Matteo, Giorgetti, Chiara, Nobile, Stefano, Ferrini, Ilaria, Simonato, Manuela, Verlato, Giovanna, Cogo, Paola, Carnielli, Virgilio Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829158/
https://www.ncbi.nlm.nih.gov/pubmed/27070307
http://dx.doi.org/10.1371/journal.pone.0153328
_version_ 1782426707547914240
author Nespeca, Matteo
Giorgetti, Chiara
Nobile, Stefano
Ferrini, Ilaria
Simonato, Manuela
Verlato, Giovanna
Cogo, Paola
Carnielli, Virgilio Paolo
author_facet Nespeca, Matteo
Giorgetti, Chiara
Nobile, Stefano
Ferrini, Ilaria
Simonato, Manuela
Verlato, Giovanna
Cogo, Paola
Carnielli, Virgilio Paolo
author_sort Nespeca, Matteo
collection PubMed
description BACKGROUND: It is unknown whether Whole-Body Hypothermia (WBH) affects pulmonary function. In vitro studies, at relatively low temperatures, suggest that hypothermia may induce significant changes to the surfactant composition. The effect of WBH on surfactant kinetics in newborn infants is unknown. We studied in vivo kinetics of disaturated-phosphatidylcholine (DSPC) in asphyxiated newborns during WBH and in normothermic controls (NTC) with no or mild asphyxia. Both groups presented no clinically apparent lung disease. METHODS: Twenty-seven term or near term newborns requiring mechanical ventilation were studied (GA 38.6±2.2 wks). Fifteen during WBH and twelve NTC. All infants received an intra-tracheal dose of (13)C labelled DSPC and tracheal aspirate were performed. DSPC amount, DSPC half-life (HL) and pool size (PS) were calculated. RESULTS: DSPC amount in tracheal aspirates was 0.42 [0.22–0.54] and 0.36 [0.10–0.58] mg/ml in WBH and NTC respectively (p = 0.578). DSPC HL was 24.9 [15.7–52.5] and 25.3 [15.8–59.3] h (p = 0.733) and DSPC PS was 53.2 [29.4–91.6] and 40.2 [29.8–64.6] mg/kg (p = 0.598) in WBH and NTC respectively. CONCLUSIONS: WBH does not alter DSPC HL and PS in newborn infants with no clinical apparent lung disease.
format Online
Article
Text
id pubmed-4829158
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48291582016-04-22 Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics? Nespeca, Matteo Giorgetti, Chiara Nobile, Stefano Ferrini, Ilaria Simonato, Manuela Verlato, Giovanna Cogo, Paola Carnielli, Virgilio Paolo PLoS One Research Article BACKGROUND: It is unknown whether Whole-Body Hypothermia (WBH) affects pulmonary function. In vitro studies, at relatively low temperatures, suggest that hypothermia may induce significant changes to the surfactant composition. The effect of WBH on surfactant kinetics in newborn infants is unknown. We studied in vivo kinetics of disaturated-phosphatidylcholine (DSPC) in asphyxiated newborns during WBH and in normothermic controls (NTC) with no or mild asphyxia. Both groups presented no clinically apparent lung disease. METHODS: Twenty-seven term or near term newborns requiring mechanical ventilation were studied (GA 38.6±2.2 wks). Fifteen during WBH and twelve NTC. All infants received an intra-tracheal dose of (13)C labelled DSPC and tracheal aspirate were performed. DSPC amount, DSPC half-life (HL) and pool size (PS) were calculated. RESULTS: DSPC amount in tracheal aspirates was 0.42 [0.22–0.54] and 0.36 [0.10–0.58] mg/ml in WBH and NTC respectively (p = 0.578). DSPC HL was 24.9 [15.7–52.5] and 25.3 [15.8–59.3] h (p = 0.733) and DSPC PS was 53.2 [29.4–91.6] and 40.2 [29.8–64.6] mg/kg (p = 0.598) in WBH and NTC respectively. CONCLUSIONS: WBH does not alter DSPC HL and PS in newborn infants with no clinical apparent lung disease. Public Library of Science 2016-04-12 /pmc/articles/PMC4829158/ /pubmed/27070307 http://dx.doi.org/10.1371/journal.pone.0153328 Text en © 2016 Nespeca et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nespeca, Matteo
Giorgetti, Chiara
Nobile, Stefano
Ferrini, Ilaria
Simonato, Manuela
Verlato, Giovanna
Cogo, Paola
Carnielli, Virgilio Paolo
Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title_full Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title_fullStr Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title_full_unstemmed Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title_short Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
title_sort does whole-body hypothermia in neonates with hypoxic–ischemic encephalopathy affect surfactant disaturated-phosphatidylcholine kinetics?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829158/
https://www.ncbi.nlm.nih.gov/pubmed/27070307
http://dx.doi.org/10.1371/journal.pone.0153328
work_keys_str_mv AT nespecamatteo doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT giorgettichiara doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT nobilestefano doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT ferriniilaria doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT simonatomanuela doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT verlatogiovanna doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT cogopaola doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics
AT carniellivirgiliopaolo doeswholebodyhypothermiainneonateswithhypoxicischemicencephalopathyaffectsurfactantdisaturatedphosphatidylcholinekinetics