Cargando…
MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine
Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. He...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829180/ https://www.ncbi.nlm.nih.gov/pubmed/27071035 http://dx.doi.org/10.1371/journal.pone.0153130 |
_version_ | 1782426710071836672 |
---|---|
author | Sato, Akira Omi, Takuya Yamamoto, Akihiro Satake, Akito Hiramoto, Akiko Masutani, Mitsuko Tanuma, Sei-ichi Wataya, Yusuke Kim, Hye-Sook |
author_facet | Sato, Akira Omi, Takuya Yamamoto, Akihiro Satake, Akito Hiramoto, Akiko Masutani, Mitsuko Tanuma, Sei-ichi Wataya, Yusuke Kim, Hye-Sook |
author_sort | Sato, Akira |
collection | PubMed |
description | Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351’s regulatory action is discussed. |
format | Online Article Text |
id | pubmed-4829180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48291802016-04-22 MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine Sato, Akira Omi, Takuya Yamamoto, Akihiro Satake, Akito Hiramoto, Akiko Masutani, Mitsuko Tanuma, Sei-ichi Wataya, Yusuke Kim, Hye-Sook PLoS One Research Article Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351’s regulatory action is discussed. Public Library of Science 2016-04-12 /pmc/articles/PMC4829180/ /pubmed/27071035 http://dx.doi.org/10.1371/journal.pone.0153130 Text en © 2016 Sato et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sato, Akira Omi, Takuya Yamamoto, Akihiro Satake, Akito Hiramoto, Akiko Masutani, Mitsuko Tanuma, Sei-ichi Wataya, Yusuke Kim, Hye-Sook MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title | MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title_full | MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title_fullStr | MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title_full_unstemmed | MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title_short | MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine |
title_sort | microrna-351 regulates two-types of cell death, necrosis and apoptosis, induced by 5-fluoro-2'-deoxyuridine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829180/ https://www.ncbi.nlm.nih.gov/pubmed/27071035 http://dx.doi.org/10.1371/journal.pone.0153130 |
work_keys_str_mv | AT satoakira microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT omitakuya microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT yamamotoakihiro microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT satakeakito microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT hiramotoakiko microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT masutanimitsuko microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT tanumaseiichi microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT watayayusuke microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine AT kimhyesook microrna351regulatestwotypesofcelldeathnecrosisandapoptosisinducedby5fluoro2deoxyuridine |