Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors

Serotonin (5-HT) modulates both neural and immune responses in vertebrates, but its role in insect immunity remains uncertain. We report that hemocytes in the caterpillar, Pieris rapae are able to synthesize 5-HT following activation by lipopolysaccharide. The inhibition of a serotonin-generating en...

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Autores principales: Qi, Yi-xiang, Huang, Jia, Li, Meng-qi, Wu, Ya-su, Xia, Ren-ying, Ye, Gong-yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829436/
https://www.ncbi.nlm.nih.gov/pubmed/26974346
http://dx.doi.org/10.7554/eLife.12241
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author Qi, Yi-xiang
Huang, Jia
Li, Meng-qi
Wu, Ya-su
Xia, Ren-ying
Ye, Gong-yin
author_facet Qi, Yi-xiang
Huang, Jia
Li, Meng-qi
Wu, Ya-su
Xia, Ren-ying
Ye, Gong-yin
author_sort Qi, Yi-xiang
collection PubMed
description Serotonin (5-HT) modulates both neural and immune responses in vertebrates, but its role in insect immunity remains uncertain. We report that hemocytes in the caterpillar, Pieris rapae are able to synthesize 5-HT following activation by lipopolysaccharide. The inhibition of a serotonin-generating enzyme with either pharmacological blockade or RNAi knock-down impaired hemocyte phagocytosis. Biochemical and functional experiments showed that naive hemocytes primarily express 5-HT(1B) and 5-HT(2B) receptors. The blockade of 5-HT(1B) significantly reduced phagocytic ability; however, the blockade of 5-HT(2B) increased hemocyte phagocytosis. The 5-HT(1B)-null Drosophila melanogaster mutants showed higher mortality than controls when infected with bacteria, due to their decreased phagocytotic ability. Flies expressing 5-HT(1B) or 5-HT(2B) RNAi in hemocytes also showed similar sensitivity to infection. Combined, these data demonstrate that 5-HT mediates hemocyte phagocytosis through 5-HT(1B) and 5-HT(2B) receptors and serotonergic signaling performs critical modulatory functions in immune systems of animals separated by 500 million years of evolution. DOI: http://dx.doi.org/10.7554/eLife.12241.001
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spelling pubmed-48294362016-04-15 Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors Qi, Yi-xiang Huang, Jia Li, Meng-qi Wu, Ya-su Xia, Ren-ying Ye, Gong-yin eLife Immunology Serotonin (5-HT) modulates both neural and immune responses in vertebrates, but its role in insect immunity remains uncertain. We report that hemocytes in the caterpillar, Pieris rapae are able to synthesize 5-HT following activation by lipopolysaccharide. The inhibition of a serotonin-generating enzyme with either pharmacological blockade or RNAi knock-down impaired hemocyte phagocytosis. Biochemical and functional experiments showed that naive hemocytes primarily express 5-HT(1B) and 5-HT(2B) receptors. The blockade of 5-HT(1B) significantly reduced phagocytic ability; however, the blockade of 5-HT(2B) increased hemocyte phagocytosis. The 5-HT(1B)-null Drosophila melanogaster mutants showed higher mortality than controls when infected with bacteria, due to their decreased phagocytotic ability. Flies expressing 5-HT(1B) or 5-HT(2B) RNAi in hemocytes also showed similar sensitivity to infection. Combined, these data demonstrate that 5-HT mediates hemocyte phagocytosis through 5-HT(1B) and 5-HT(2B) receptors and serotonergic signaling performs critical modulatory functions in immune systems of animals separated by 500 million years of evolution. DOI: http://dx.doi.org/10.7554/eLife.12241.001 eLife Sciences Publications, Ltd 2016-03-14 /pmc/articles/PMC4829436/ /pubmed/26974346 http://dx.doi.org/10.7554/eLife.12241 Text en © 2016, Qi et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Immunology
Qi, Yi-xiang
Huang, Jia
Li, Meng-qi
Wu, Ya-su
Xia, Ren-ying
Ye, Gong-yin
Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title_full Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title_fullStr Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title_full_unstemmed Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title_short Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
title_sort serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829436/
https://www.ncbi.nlm.nih.gov/pubmed/26974346
http://dx.doi.org/10.7554/eLife.12241
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