A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan

Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to s...

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Autores principales: OBA, Mami, OMATSU, Tsutomu, TAKANO, Ai, FUJITA, Hiromi, SATO, Kozue, NAKAMOTO, Atsushi, TAKAHASHI, Mamoru, TAKADA, Nobuhiro, KAWABATA, Hiroki, ANDO, Shuji, MIZUTANI, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829514/
https://www.ncbi.nlm.nih.gov/pubmed/26498534
http://dx.doi.org/10.1292/jvms.15-0536
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author OBA, Mami
OMATSU, Tsutomu
TAKANO, Ai
FUJITA, Hiromi
SATO, Kozue
NAKAMOTO, Atsushi
TAKAHASHI, Mamoru
TAKADA, Nobuhiro
KAWABATA, Hiroki
ANDO, Shuji
MIZUTANI, Tetsuya
author_facet OBA, Mami
OMATSU, Tsutomu
TAKANO, Ai
FUJITA, Hiromi
SATO, Kozue
NAKAMOTO, Atsushi
TAKAHASHI, Mamoru
TAKADA, Nobuhiro
KAWABATA, Hiroki
ANDO, Shuji
MIZUTANI, Tetsuya
author_sort OBA, Mami
collection PubMed
description Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to segments of Bunyaviruses, Issyk-Kul virus. The identities of segment L, M and S were only 77, 76 and 79% to Issyk-Kul virus, respectively. Therefore, we named this novel virus Soft tick bunyavirus (STBV). In the phylogenetic tree, segment L of STBV was closely related to a cluster consisting of the genus Nairovirus of the family Bunyaviridae.
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spelling pubmed-48295142016-04-13 A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan OBA, Mami OMATSU, Tsutomu TAKANO, Ai FUJITA, Hiromi SATO, Kozue NAKAMOTO, Atsushi TAKAHASHI, Mamoru TAKADA, Nobuhiro KAWABATA, Hiroki ANDO, Shuji MIZUTANI, Tetsuya J Vet Med Sci Virology Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to segments of Bunyaviruses, Issyk-Kul virus. The identities of segment L, M and S were only 77, 76 and 79% to Issyk-Kul virus, respectively. Therefore, we named this novel virus Soft tick bunyavirus (STBV). In the phylogenetic tree, segment L of STBV was closely related to a cluster consisting of the genus Nairovirus of the family Bunyaviridae. The Japanese Society of Veterinary Science 2015-10-25 2016-03 /pmc/articles/PMC4829514/ /pubmed/26498534 http://dx.doi.org/10.1292/jvms.15-0536 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
OBA, Mami
OMATSU, Tsutomu
TAKANO, Ai
FUJITA, Hiromi
SATO, Kozue
NAKAMOTO, Atsushi
TAKAHASHI, Mamoru
TAKADA, Nobuhiro
KAWABATA, Hiroki
ANDO, Shuji
MIZUTANI, Tetsuya
A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title_full A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title_fullStr A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title_full_unstemmed A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title_short A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan
title_sort novel bunyavirus from the soft tick, argas vespertilionis, in japan
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829514/
https://www.ncbi.nlm.nih.gov/pubmed/26498534
http://dx.doi.org/10.1292/jvms.15-0536
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