Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra

Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of b...

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Autores principales: TAMADA, Hiromichi, ADACHI, Nahoko, KAWATE, Noritoshi, INABA, Toshio, HATOYA, Shingo, SAWADA, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Theriogenology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829531/
https://www.ncbi.nlm.nih.gov/pubmed/26596635
http://dx.doi.org/10.1292/jvms.15-0520
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author TAMADA, Hiromichi
ADACHI, Nahoko
KAWATE, Noritoshi
INABA, Toshio
HATOYA, Shingo
SAWADA, Tsutomu
author_facet TAMADA, Hiromichi
ADACHI, Nahoko
KAWATE, Noritoshi
INABA, Toshio
HATOYA, Shingo
SAWADA, Tsutomu
author_sort TAMADA, Hiromichi
collection PubMed
description Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of bitches with pyometra. Cervical patency was measured by inserting the stainless steel rods with different diameter into cervical canals. Levels of mRNA expression were determined by semi-quantitative reverse transcription-polymerase chain reaction. The cervical patency was positively correlated with mRNA levels for COX-2 and PGES, but not those for iNOS and COX-1. The results suggest that gene expression of COX-2 and PGES may be involved in the regulation of patency in the uterine cervix of bitches with pyometra.
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spelling pubmed-48295312016-04-13 Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra TAMADA, Hiromichi ADACHI, Nahoko KAWATE, Noritoshi INABA, Toshio HATOYA, Shingo SAWADA, Tsutomu J Vet Med Sci Theriogenology Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of bitches with pyometra. Cervical patency was measured by inserting the stainless steel rods with different diameter into cervical canals. Levels of mRNA expression were determined by semi-quantitative reverse transcription-polymerase chain reaction. The cervical patency was positively correlated with mRNA levels for COX-2 and PGES, but not those for iNOS and COX-1. The results suggest that gene expression of COX-2 and PGES may be involved in the regulation of patency in the uterine cervix of bitches with pyometra. The Japanese Society of Veterinary Science 2015-11-22 2016-03 /pmc/articles/PMC4829531/ /pubmed/26596635 http://dx.doi.org/10.1292/jvms.15-0520 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Theriogenology
TAMADA, Hiromichi
ADACHI, Nahoko
KAWATE, Noritoshi
INABA, Toshio
HATOYA, Shingo
SAWADA, Tsutomu
Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title_full Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title_fullStr Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title_full_unstemmed Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title_short Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra
title_sort positive correlation between patency and mrna levels for cyclooxygenase-2 and prostaglandin e synthase in the uterine cervix of bitches with pyometra
topic Theriogenology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829531/
https://www.ncbi.nlm.nih.gov/pubmed/26596635
http://dx.doi.org/10.1292/jvms.15-0520
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