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Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish

The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles int...

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Autores principales: Brundo, Maria V., Pecoraro, Roberta, Marino, Fabio, Salvaggio, Antonio, Tibullo, Daniele, Saccone, Salvatore, Bramanti, Vincenzo, Buccheri, Maria A., Impellizzeri, Giuliana, Scuderi, Viviana, Zimbone, Massimo, Privitera, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829578/
https://www.ncbi.nlm.nih.gov/pubmed/27148069
http://dx.doi.org/10.3389/fphys.2016.00130
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author Brundo, Maria V.
Pecoraro, Roberta
Marino, Fabio
Salvaggio, Antonio
Tibullo, Daniele
Saccone, Salvatore
Bramanti, Vincenzo
Buccheri, Maria A.
Impellizzeri, Giuliana
Scuderi, Viviana
Zimbone, Massimo
Privitera, Vittorio
author_facet Brundo, Maria V.
Pecoraro, Roberta
Marino, Fabio
Salvaggio, Antonio
Tibullo, Daniele
Saccone, Salvatore
Bramanti, Vincenzo
Buccheri, Maria A.
Impellizzeri, Giuliana
Scuderi, Viviana
Zimbone, Massimo
Privitera, Vittorio
author_sort Brundo, Maria V.
collection PubMed
description The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles into new nanomaterials. The nanomaterials tested were synthesized proposing a ground-breaking approach by an upside-down vision of the Au/TiO(2) nano-system to avoid the release of nanoparticles. The system was synthesized by wrapping Au nanoparticles with a thin layer of TiO(2). The non-toxicity of the nano-system was established by testing the effect of the material on zebrafish larvae. Danio rerio o zebrafish was considered an excellent model for the environmental biomonitoring of aquatic environments and the Zebrafish Embryo Toxicity Test (ZFET) is considered an alternative method of animal test. For this reason zebrafish larvae were exposed to different concentrations of nanoparticles of TiO(2) and Au and new nanomaterials. As biomarkers of exposure, we evaluated the expression of metallothioneins by immunohistochemistry analysis and western blotting analysis also. The results obtained by toxicity test showed that neither mortality as well as sublethal effects were induced by the different nanomaterials and nanoparticles tested. Only zebrafish larvae exposed to free Au nanoparticles showed a different response to anti-MT antibody. In fact, the immunolocalization analysis highlighted an increase of the metallothioneins synthesis.
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spelling pubmed-48295782016-05-04 Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish Brundo, Maria V. Pecoraro, Roberta Marino, Fabio Salvaggio, Antonio Tibullo, Daniele Saccone, Salvatore Bramanti, Vincenzo Buccheri, Maria A. Impellizzeri, Giuliana Scuderi, Viviana Zimbone, Massimo Privitera, Vittorio Front Physiol Physiology The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles into new nanomaterials. The nanomaterials tested were synthesized proposing a ground-breaking approach by an upside-down vision of the Au/TiO(2) nano-system to avoid the release of nanoparticles. The system was synthesized by wrapping Au nanoparticles with a thin layer of TiO(2). The non-toxicity of the nano-system was established by testing the effect of the material on zebrafish larvae. Danio rerio o zebrafish was considered an excellent model for the environmental biomonitoring of aquatic environments and the Zebrafish Embryo Toxicity Test (ZFET) is considered an alternative method of animal test. For this reason zebrafish larvae were exposed to different concentrations of nanoparticles of TiO(2) and Au and new nanomaterials. As biomarkers of exposure, we evaluated the expression of metallothioneins by immunohistochemistry analysis and western blotting analysis also. The results obtained by toxicity test showed that neither mortality as well as sublethal effects were induced by the different nanomaterials and nanoparticles tested. Only zebrafish larvae exposed to free Au nanoparticles showed a different response to anti-MT antibody. In fact, the immunolocalization analysis highlighted an increase of the metallothioneins synthesis. Frontiers Media S.A. 2016-04-13 /pmc/articles/PMC4829578/ /pubmed/27148069 http://dx.doi.org/10.3389/fphys.2016.00130 Text en Copyright © 2016 Brundo, Pecoraro, Marino, Salvaggio, Tibullo, Saccone, Bramanti, Buccheri, Impellizzeri, Scuderi, Zimbone and Privitera. http://creativecommons.org/licenses/by/4.0/ Copyright © 2016 Brundo, Pecoraro, Marino, Salvaggio, Tibullo, Saccone, Bramanti, Buccheri, Impellizzeri, Scuderi, Zimbone and Privitera. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Brundo, Maria V.
Pecoraro, Roberta
Marino, Fabio
Salvaggio, Antonio
Tibullo, Daniele
Saccone, Salvatore
Bramanti, Vincenzo
Buccheri, Maria A.
Impellizzeri, Giuliana
Scuderi, Viviana
Zimbone, Massimo
Privitera, Vittorio
Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title_full Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title_fullStr Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title_full_unstemmed Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title_short Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish
title_sort toxicity evaluation of new engineered nanomaterials in zebrafish
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829578/
https://www.ncbi.nlm.nih.gov/pubmed/27148069
http://dx.doi.org/10.3389/fphys.2016.00130
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