Cargando…

Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum

A high density genetic map was constructed using F(2) population derived from an interspecific cross of G. hirsutum × G. tomentosum. The map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cM of cotton genome with an average inter-marker distance of 1.48 cM...

Descripción completa

Detalles Bibliográficos
Autores principales: Khan, Muhammad K. R., Chen, Haodong, Zhou, Zhongli, Ilyas, Muhammad K., Wang, Xingxing, Cai, Xiaoyan, Wang, Chunying, Liu, Fang, Wang, Kunbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829609/
https://www.ncbi.nlm.nih.gov/pubmed/27148280
http://dx.doi.org/10.3389/fpls.2016.00436
_version_ 1782426769919311872
author Khan, Muhammad K. R.
Chen, Haodong
Zhou, Zhongli
Ilyas, Muhammad K.
Wang, Xingxing
Cai, Xiaoyan
Wang, Chunying
Liu, Fang
Wang, Kunbo
author_facet Khan, Muhammad K. R.
Chen, Haodong
Zhou, Zhongli
Ilyas, Muhammad K.
Wang, Xingxing
Cai, Xiaoyan
Wang, Chunying
Liu, Fang
Wang, Kunbo
author_sort Khan, Muhammad K. R.
collection PubMed
description A high density genetic map was constructed using F(2) population derived from an interspecific cross of G. hirsutum × G. tomentosum. The map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cM of cotton genome with an average inter-marker distance of 1.48 cM. The maximum length of chromosome was 218.38 cM and the minimum was 122.09 cM with an average length of 167.90 cM. A sub-genome covers more genetic distance (2189.01 cM) with an average inter loci distance of 1.53 cM than D sub-genome which covers a length of 2176.29 cM with an average distance of 1.43 cM. There were 716 distorted loci in the map accounting for 23.14% and most distorted loci were distributed on D sub-genome (25.06%), which were more than on A sub-genome (21.23%). In our map 49 segregation hotspots (SDR) were distributed across the genome with more on D sub-genome as compared to A genome. Two post-polyploidization reciprocal translocations of “A2/A3 and A4/A5” were suggested by seven pairs of duplicate loci. The map constructed through these studies is one of the three densest genetic maps in cotton however; this is the first dense genome wide SSR interspecific genetic map between G. hirsutum and G. tomentosum.
format Online
Article
Text
id pubmed-4829609
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-48296092016-05-04 Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum Khan, Muhammad K. R. Chen, Haodong Zhou, Zhongli Ilyas, Muhammad K. Wang, Xingxing Cai, Xiaoyan Wang, Chunying Liu, Fang Wang, Kunbo Front Plant Sci Plant Science A high density genetic map was constructed using F(2) population derived from an interspecific cross of G. hirsutum × G. tomentosum. The map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cM of cotton genome with an average inter-marker distance of 1.48 cM. The maximum length of chromosome was 218.38 cM and the minimum was 122.09 cM with an average length of 167.90 cM. A sub-genome covers more genetic distance (2189.01 cM) with an average inter loci distance of 1.53 cM than D sub-genome which covers a length of 2176.29 cM with an average distance of 1.43 cM. There were 716 distorted loci in the map accounting for 23.14% and most distorted loci were distributed on D sub-genome (25.06%), which were more than on A sub-genome (21.23%). In our map 49 segregation hotspots (SDR) were distributed across the genome with more on D sub-genome as compared to A genome. Two post-polyploidization reciprocal translocations of “A2/A3 and A4/A5” were suggested by seven pairs of duplicate loci. The map constructed through these studies is one of the three densest genetic maps in cotton however; this is the first dense genome wide SSR interspecific genetic map between G. hirsutum and G. tomentosum. Frontiers Media S.A. 2016-04-13 /pmc/articles/PMC4829609/ /pubmed/27148280 http://dx.doi.org/10.3389/fpls.2016.00436 Text en Copyright © 2016 Khan, Chen, Zhou, Ilyas, Wang, Cai, Wang, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Khan, Muhammad K. R.
Chen, Haodong
Zhou, Zhongli
Ilyas, Muhammad K.
Wang, Xingxing
Cai, Xiaoyan
Wang, Chunying
Liu, Fang
Wang, Kunbo
Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title_full Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title_fullStr Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title_full_unstemmed Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title_short Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum
title_sort genome wide ssr high density genetic map construction from an interspecific cross of gossypium hirsutum × gossypium tomentosum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829609/
https://www.ncbi.nlm.nih.gov/pubmed/27148280
http://dx.doi.org/10.3389/fpls.2016.00436
work_keys_str_mv AT khanmuhammadkr genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT chenhaodong genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT zhouzhongli genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT ilyasmuhammadk genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT wangxingxing genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT caixiaoyan genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT wangchunying genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT liufang genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum
AT wangkunbo genomewidessrhighdensitygeneticmapconstructionfromaninterspecificcrossofgossypiumhirsutumgossypiumtomentosum