Cargando…

4D Visualization of replication foci in mammalian cells corresponding to individual replicons

Since the pioneering proposal of the replicon model of DNA replication 50 years ago, the predicted replicons have not been identified and quantified at the cellular level. Here, we combine conventional and super-resolution microscopy of replication sites in live and fixed cells with computational im...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chagin, V. O., Casas-Delucchi, C. S., Reinhart, M., Schermelleh, L., Markaki, Y., Maiser, A., Bolius, J. J., Bensimon, A., Fillies, M., Domaing, P., Rozanov, Y. M., Leonhardt, H., Cardoso, M. C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829660/ https://www.ncbi.nlm.nih.gov/pubmed/27052570 http://dx.doi.org/10.1038/ncomms11231

Ejemplares similares

3D replicon distributions arise from stochastic initiation and domino-like DNA replication progression
por: Löb, D., et al.
Publicado: (2016)

Developmental differences in genome replication program and origin activation
por: Rausch, Cathia, et al.
Publicado: (2020)

Measurement of replication structures at the nanometer scale using super-resolution light microscopy
por: Baddeley, D., et al.
Publicado: (2010)

Targeted manipulation of heterochromatin rescues MeCP2 Rett mutants and re-establishes higher order chromatin organization
por: Casas-Delucchi, Corella S., et al.
Publicado: (2012)

Establishment and mitotic stability of an extra-chromosomal mammalian replicon
por: Stehle, Isa M, et al.
Publicado: (2007)

Super-resolution in situ analysis of active ribosomal DNA chromatin organization in the nucleolus
por: Maiser, Andreas, et al.
Publicado: (2020)

PCNA acts as a stationary loading platform for transiently interacting Okazaki fragment maturation proteins
por: Sporbert, Anje, et al.
Publicado: (2005)

Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci
por: Smeets, Daniel, et al.
Publicado: (2014)

Facile method for delivering chikungunya viral replicons into mosquitoes and mammalian cells
por: Lin, Hui-Chung, et al.
Publicado: (2021)

Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells
por: Krammer, Florian, et al.
Publicado: (2010)

Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
por: Colunga-Saucedo, Mayra, et al.
Publicado: (2022)

Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair
por: Chagin, Vadim O., et al.
Publicado: (2019)

Cdc45 Limits Replicon Usage from a Low Density of preRCs in Mammalian Cells
por: Wong, Philip G., et al.
Publicado: (2011)

The Adjuvant Activity of Alphavirus Replicons Is Enhanced by Incorporating the Microbial Molecule Flagellin into the Replicon
por: Knudsen, Maria L., et al.
Publicado: (2013)

Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters
por: Cremer, Marion, et al.
Publicado: (2017)

Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y
por: Wiedemann, Sonja M., et al.
Publicado: (2010)

Histone acetylation controls the inactive X chromosome replication dynamics
por: Casas-Delucchi, Corella S., et al.
Publicado: (2011)

Replicon RNA Viral Vectors as Vaccines
por: Lundstrom, Kenneth
Publicado: (2016)

Zika Virus Replicons for Drug Discovery
por: Xie, Xuping, et al.
Publicado: (2016)

Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming
por: Grohmann, Maik, et al.
Publicado: (2005)

New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions
por: Herce, HD, et al.
Publicado: (2013)

Development and characterization of promoterless helper RNAs for the production of alphavirus replicon particle
por: Kamrud, K. I., et al.
Publicado: (2010)

Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells
por: Kilgas, Susan, et al.
Publicado: (2021)

Independent origins and evolution of the secondary replicons of the class Gammaproteobacteria
por: Riccardi, Christopher, et al.
Publicado: (2023)

A guide to super-resolution fluorescence microscopy
por: Schermelleh, Lothar, et al.
Publicado: (2010)

Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells
por: Okoli, Arinze S., et al.
Publicado: (2012)

Organization of human replicon: Singles or zipping couples?
por: Ligasová, Anna, et al.
Publicado: (2009)

In vitro evolution of enhanced RNA replicons for immunotherapy
por: Li, Yingzhong, et al.
Publicado: (2019)

Viral replicons as valuable tools for drug discovery
por: Hannemann, Holger
Publicado: (2020)

Evolutionary Trajectory of the Replication Mode of Bacterial Replicons
por: Xie, Bin-Bin, et al.
Publicado: (2021)

Reconstitution of a telomeric replicon organized by CST
por: Zaug, Arthur J., et al.
Publicado: (2022)

Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams
por: Carattoli, Alessandra, et al.
Publicado: (2006)

The location and development of Replicon Cluster Domains in early replicating DNA
por: da Costa-Nunes, José A., et al.
Publicado: (2023)

Pentavalent replicon vaccines against botulinum neurotoxins and tetanus toxin using DNA-based Semliki Forest virus replicon vectors
por: Yu, YunZhou, et al.
Publicado: (2014)

Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation
por: Schermelleh, Lothar, et al.
Publicado: (2007)

Genetic evidence suggests that Spata22 is required for the maintenance of Rad51 foci in mammalian meiosis
por: Ishishita, Satoshi, et al.
Publicado: (2014)

A-Type Lamins Maintain the Positional Stability of DNA Damage Repair Foci in Mammalian Nuclei
por: Mahen, Robert, et al.
Publicado: (2013)

Synthesis of New Indanyl Nucleoside Analogues and their Biological Evaluation on Hepatitis C Virus (HCV) Replicon
por: Gómez, Matías E., et al.
Publicado: (2019)

Histone hypoacetylation is required to maintain late replication timing of constitutive heterochromatin
por: Casas-Delucchi, Corella S., et al.
Publicado: (2012)

Metabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti
por: diCenzo, George C., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_nl6i3asd5u6tcip2vk60tat4v7