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Defining Clonal Color in Fluorescent Multi-Clonal Tracking
Clonal heterogeneity and selection underpin many biological processes including development and tumor progression. Combinatorial fluorescent protein expression in germline cells has proven its utility for tracking the formation and regeneration of different organ systems. Such cell populations encod...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829845/ https://www.ncbi.nlm.nih.gov/pubmed/27073117 http://dx.doi.org/10.1038/srep24303 |
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author | Wu, Juwell W. Turcotte, Raphaël Alt, Clemens Runnels, Judith M. Tsao, Hensin Lin, Charles P. |
author_facet | Wu, Juwell W. Turcotte, Raphaël Alt, Clemens Runnels, Judith M. Tsao, Hensin Lin, Charles P. |
author_sort | Wu, Juwell W. |
collection | PubMed |
description | Clonal heterogeneity and selection underpin many biological processes including development and tumor progression. Combinatorial fluorescent protein expression in germline cells has proven its utility for tracking the formation and regeneration of different organ systems. Such cell populations encoded by combinatorial fluorescent proteins are also attractive tools for understanding clonal expansion and clonal competition in cancer. However, the assignment of clonal identity requires an analytical framework in which clonal markings can be parameterized and validated. Here we present a systematic and quantitative method for RGB analysis of fluorescent melanoma cancer clones. We then demonstrate refined clonal trackability of melanoma cells using this scheme. |
format | Online Article Text |
id | pubmed-4829845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48298452016-04-19 Defining Clonal Color in Fluorescent Multi-Clonal Tracking Wu, Juwell W. Turcotte, Raphaël Alt, Clemens Runnels, Judith M. Tsao, Hensin Lin, Charles P. Sci Rep Article Clonal heterogeneity and selection underpin many biological processes including development and tumor progression. Combinatorial fluorescent protein expression in germline cells has proven its utility for tracking the formation and regeneration of different organ systems. Such cell populations encoded by combinatorial fluorescent proteins are also attractive tools for understanding clonal expansion and clonal competition in cancer. However, the assignment of clonal identity requires an analytical framework in which clonal markings can be parameterized and validated. Here we present a systematic and quantitative method for RGB analysis of fluorescent melanoma cancer clones. We then demonstrate refined clonal trackability of melanoma cells using this scheme. Nature Publishing Group 2016-04-13 /pmc/articles/PMC4829845/ /pubmed/27073117 http://dx.doi.org/10.1038/srep24303 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wu, Juwell W. Turcotte, Raphaël Alt, Clemens Runnels, Judith M. Tsao, Hensin Lin, Charles P. Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title | Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title_full | Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title_fullStr | Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title_full_unstemmed | Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title_short | Defining Clonal Color in Fluorescent Multi-Clonal Tracking |
title_sort | defining clonal color in fluorescent multi-clonal tracking |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829845/ https://www.ncbi.nlm.nih.gov/pubmed/27073117 http://dx.doi.org/10.1038/srep24303 |
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