A novel and highly sensitive nanocatalytic surface plasmon resonance-scattering analytical platform for detection of trace Pb ions

Gold nanoparticles (AuNP) have catalysis on the reaction of HAuCl(4)-H(2)O(2). The produced AuNP have strong resonance Rayleigh scattering (RRS) effect and surface-enhanced resonance Raman scattering (SERS) effect when Victoria blue B (VBB) and rhodamine S (RhS) were used as probes. The increased RRS/SERS intensity respond linearly with the concentration of gold nanoparticles (AuNP(B)) which synthesized by NaBH(4) over 0.038–76 ng/mL, 19–285 ng/mL, 3.8–456 ng/mL respectively. Four kinds of tested nanoparticles have catalysis on the HAuCl(4)-H(2)O(2) particles reaction. Thus, a novel nanocatalysis surface plasmon resonance-scattering (SPR-S) analytical platform was developed for AuNP. The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn’t protect AuNP(c) to aggregate to AuNP(c) aggregations, having strong RRS effect. Upon addition of Pb(2+), dsDNA could be cracked by Pb(2+) to produce single-stranded DNA (ssDNA) that adsorbed on the AuNPc surface to form AuNPc-ssDNA conjugates. The conjugates have strong catalysis on HAuCl(4)-H(2)O(2) reaction. With increased Pb(2+) concentration, the concentration of AuNPc-ssDNA increased and lead to the catalytic activity stronger. The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7–666.7 nmol/L. The SERS intensity responded linearly with the concentration of Pb(2+) over 50–500 nmol/L.