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Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST)
BACKGROUND: Osteoarthritis (OA) is characterized by progressive loss of cartilage in joints, and is a major cause of pain and disability, and imposes significant health care expense. New therapies are being developed to treat the symptomatic effect of OA, one of which is intra-articular injection of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830049/ https://www.ncbi.nlm.nih.gov/pubmed/27071650 http://dx.doi.org/10.1186/s12967-016-0850-8 |
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author | Haris, Mohammad Singh, Anup Reddy, Sanjana Bagga, Puneet Kneeland, J. Bruce Tjoumakaris, Fotios P. Hariharan, Hari Marincola, Francesco M. Reddy, Ravinder |
author_facet | Haris, Mohammad Singh, Anup Reddy, Sanjana Bagga, Puneet Kneeland, J. Bruce Tjoumakaris, Fotios P. Hariharan, Hari Marincola, Francesco M. Reddy, Ravinder |
author_sort | Haris, Mohammad |
collection | PubMed |
description | BACKGROUND: Osteoarthritis (OA) is characterized by progressive loss of cartilage in joints, and is a major cause of pain and disability, and imposes significant health care expense. New therapies are being developed to treat the symptomatic effect of OA, one of which is intra-articular injection of viscosupplementations of different forms of hyaluronic acid (HA). The current study evaluates the chemical exchange saturation transfer (CEST) effect from two popular viscosupplementations [Hylan gf-20 (Synvisc) and hyaluronan (Orthovisc)] by targeting the exchangeable hydroxyl protons present on these molecules (ViscoCEST). METHODS: ViscoCEST imaging from two viscosupplementations (Synvisc and Orthovisc) was performed on a 7T Siemens whole body MRI scanner. ViscoCEST images were collected with different combination of saturation pulse power and saturation duration. Z spectra were acquired at B(1rms) of 3.6 μT and 1 s saturation duration by varying the frequency from −4 to +4 ppm in step size of 0.1 ppm. Field inhomogeneity (B(0)) and radiofrequency (B(1)) maps were also acquired to correct ViscoCEST contrast map for any inhomogeneity. RESULTS: Both viscosupplementations showed broad CEST effect (ViscoCEST), which peaked ~0.8 ppm from down field of water resonance. Orthovisc showed 20 % higher ViscoCEST contrast than Synvisc suggestive of more HA component in Orthovisc. Increased ViscoCEST contrast was observed from both viscosupplementations with increase in B(1rms) and saturation pulse duration. CONCLUSION: ViscoCEST has a potential to image the spatial distribution of viscosupplements in vivo in patients’ intra-articular space as well as temporal variation in their spatial distribution. |
format | Online Article Text |
id | pubmed-4830049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48300492016-04-14 Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) Haris, Mohammad Singh, Anup Reddy, Sanjana Bagga, Puneet Kneeland, J. Bruce Tjoumakaris, Fotios P. Hariharan, Hari Marincola, Francesco M. Reddy, Ravinder J Transl Med Research BACKGROUND: Osteoarthritis (OA) is characterized by progressive loss of cartilage in joints, and is a major cause of pain and disability, and imposes significant health care expense. New therapies are being developed to treat the symptomatic effect of OA, one of which is intra-articular injection of viscosupplementations of different forms of hyaluronic acid (HA). The current study evaluates the chemical exchange saturation transfer (CEST) effect from two popular viscosupplementations [Hylan gf-20 (Synvisc) and hyaluronan (Orthovisc)] by targeting the exchangeable hydroxyl protons present on these molecules (ViscoCEST). METHODS: ViscoCEST imaging from two viscosupplementations (Synvisc and Orthovisc) was performed on a 7T Siemens whole body MRI scanner. ViscoCEST images were collected with different combination of saturation pulse power and saturation duration. Z spectra were acquired at B(1rms) of 3.6 μT and 1 s saturation duration by varying the frequency from −4 to +4 ppm in step size of 0.1 ppm. Field inhomogeneity (B(0)) and radiofrequency (B(1)) maps were also acquired to correct ViscoCEST contrast map for any inhomogeneity. RESULTS: Both viscosupplementations showed broad CEST effect (ViscoCEST), which peaked ~0.8 ppm from down field of water resonance. Orthovisc showed 20 % higher ViscoCEST contrast than Synvisc suggestive of more HA component in Orthovisc. Increased ViscoCEST contrast was observed from both viscosupplementations with increase in B(1rms) and saturation pulse duration. CONCLUSION: ViscoCEST has a potential to image the spatial distribution of viscosupplements in vivo in patients’ intra-articular space as well as temporal variation in their spatial distribution. BioMed Central 2016-04-12 /pmc/articles/PMC4830049/ /pubmed/27071650 http://dx.doi.org/10.1186/s12967-016-0850-8 Text en © Haris et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Haris, Mohammad Singh, Anup Reddy, Sanjana Bagga, Puneet Kneeland, J. Bruce Tjoumakaris, Fotios P. Hariharan, Hari Marincola, Francesco M. Reddy, Ravinder Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title | Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title_full | Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title_fullStr | Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title_full_unstemmed | Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title_short | Characterization of viscosupplementation formulations using chemical exchange saturation transfer (ViscoCEST) |
title_sort | characterization of viscosupplementation formulations using chemical exchange saturation transfer (viscocest) |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830049/ https://www.ncbi.nlm.nih.gov/pubmed/27071650 http://dx.doi.org/10.1186/s12967-016-0850-8 |
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