Cargando…

Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects

BACKGROUND: Hybrid breakdown has been well documented in various species. Relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. But correlations between genomic heterozygosity and vegetative and reproductive traits in co...

Descripción completa

Detalles Bibliográficos
Autores principales: Dai, Baosheng, Guo, Huanle, Huang, Cong, Zhang, Xianlong, Lin, Zhongxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830075/
https://www.ncbi.nlm.nih.gov/pubmed/27072350
http://dx.doi.org/10.1186/s12863-016-0366-5
_version_ 1782426854467043328
author Dai, Baosheng
Guo, Huanle
Huang, Cong
Zhang, Xianlong
Lin, Zhongxu
author_facet Dai, Baosheng
Guo, Huanle
Huang, Cong
Zhang, Xianlong
Lin, Zhongxu
author_sort Dai, Baosheng
collection PubMed
description BACKGROUND: Hybrid breakdown has been well documented in various species. Relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. But correlations between genomic heterozygosity and vegetative and reproductive traits in cotton interspecific populations have not been studied. In the current study, two reciprocal F(2) populations were developed using Gossypium hirsutum cv. Emian 22 and G. barbadense acc. 3–79 as parents to study hybrid breakdown in cotton. A total of 125 simple sequence repeat (SSR) markers were used to genotype the two F(2) interspecific populations. RESULTS: To guarantee mutual independence among the genotyped markers, the 125 SSR markers were checked by the linkage disequilibrium analysis. To our knowledge, this is a novel approach to evaluate the individual genomic heterozygosity. After marker checking, 83 common loci were used to assess the extent of genomic heterozygosity. Hybrid breakdown was found extensively in the two interspecific F(2) populations particularly on the reproductive traits because of the infertility and the bare seeds. And then, the relationships between the genomic heterozygosity and the vegetative reproductive traits were investigated. The only relationships between hybrid breakdown and heterozygosity were observed in the (Emian22 × 3–79) F(2) population for seed index (SI) and boll number per plant (BN). The maternal cytoplasmic environment may have a significant effect on genomic heterozygosity and on correlations between heterozygosity and reproductive traits. CONCLUSIONS: A novel approach was used to evaluate genomic heterozygosity in cotton; and hybrid breakdown was observed in reproductive traits in cotton. These findings may offer new insight into hybrid breakdown in allotetraploid cotton interspecific hybrids, and may be useful for the development of interspecific hybrids for cotton genetic improvement. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-016-0366-5) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4830075
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-48300752016-04-14 Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects Dai, Baosheng Guo, Huanle Huang, Cong Zhang, Xianlong Lin, Zhongxu BMC Genet Research Article BACKGROUND: Hybrid breakdown has been well documented in various species. Relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. But correlations between genomic heterozygosity and vegetative and reproductive traits in cotton interspecific populations have not been studied. In the current study, two reciprocal F(2) populations were developed using Gossypium hirsutum cv. Emian 22 and G. barbadense acc. 3–79 as parents to study hybrid breakdown in cotton. A total of 125 simple sequence repeat (SSR) markers were used to genotype the two F(2) interspecific populations. RESULTS: To guarantee mutual independence among the genotyped markers, the 125 SSR markers were checked by the linkage disequilibrium analysis. To our knowledge, this is a novel approach to evaluate the individual genomic heterozygosity. After marker checking, 83 common loci were used to assess the extent of genomic heterozygosity. Hybrid breakdown was found extensively in the two interspecific F(2) populations particularly on the reproductive traits because of the infertility and the bare seeds. And then, the relationships between the genomic heterozygosity and the vegetative reproductive traits were investigated. The only relationships between hybrid breakdown and heterozygosity were observed in the (Emian22 × 3–79) F(2) population for seed index (SI) and boll number per plant (BN). The maternal cytoplasmic environment may have a significant effect on genomic heterozygosity and on correlations between heterozygosity and reproductive traits. CONCLUSIONS: A novel approach was used to evaluate genomic heterozygosity in cotton; and hybrid breakdown was observed in reproductive traits in cotton. These findings may offer new insight into hybrid breakdown in allotetraploid cotton interspecific hybrids, and may be useful for the development of interspecific hybrids for cotton genetic improvement. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-016-0366-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-12 /pmc/articles/PMC4830075/ /pubmed/27072350 http://dx.doi.org/10.1186/s12863-016-0366-5 Text en © Dai et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Dai, Baosheng
Guo, Huanle
Huang, Cong
Zhang, Xianlong
Lin, Zhongxu
Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title_full Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title_fullStr Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title_full_unstemmed Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title_short Genomic heterozygosity and hybrid breakdown in cotton (Gossypium): different traits, different effects
title_sort genomic heterozygosity and hybrid breakdown in cotton (gossypium): different traits, different effects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830075/
https://www.ncbi.nlm.nih.gov/pubmed/27072350
http://dx.doi.org/10.1186/s12863-016-0366-5
work_keys_str_mv AT daibaosheng genomicheterozygosityandhybridbreakdownincottongossypiumdifferenttraitsdifferenteffects
AT guohuanle genomicheterozygosityandhybridbreakdownincottongossypiumdifferenttraitsdifferenteffects
AT huangcong genomicheterozygosityandhybridbreakdownincottongossypiumdifferenttraitsdifferenteffects
AT zhangxianlong genomicheterozygosityandhybridbreakdownincottongossypiumdifferenttraitsdifferenteffects
AT linzhongxu genomicheterozygosityandhybridbreakdownincottongossypiumdifferenttraitsdifferenteffects