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The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA
Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830116/ https://www.ncbi.nlm.nih.gov/pubmed/27074256 http://dx.doi.org/10.1590/0074-02760150466 |
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author | Barbedo, Leonardo Silva Figueiredo-Carvalho, Maria Helena Galdino Muniz, Mauro de Medeiros Zancopé-Oliveira, Rosely Maria |
author_facet | Barbedo, Leonardo Silva Figueiredo-Carvalho, Maria Helena Galdino Muniz, Mauro de Medeiros Zancopé-Oliveira, Rosely Maria |
author_sort | Barbedo, Leonardo Silva |
collection | PubMed |
description | Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories. |
format | Online Article Text |
id | pubmed-4830116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-48301162016-04-13 The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA Barbedo, Leonardo Silva Figueiredo-Carvalho, Maria Helena Galdino Muniz, Mauro de Medeiros Zancopé-Oliveira, Rosely Maria Mem Inst Oswaldo Cruz Short Communication Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories. Instituto Oswaldo Cruz, Ministério da Saúde 2016-04 /pmc/articles/PMC4830116/ /pubmed/27074256 http://dx.doi.org/10.1590/0074-02760150466 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Barbedo, Leonardo Silva Figueiredo-Carvalho, Maria Helena Galdino Muniz, Mauro de Medeiros Zancopé-Oliveira, Rosely Maria The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
title | The identification and differentiation of the Candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rDNA |
title_full | The identification and differentiation of the Candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rDNA |
title_fullStr | The identification and differentiation of the Candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rDNA |
title_full_unstemmed | The identification and differentiation of the Candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rDNA |
title_short | The identification and differentiation of the Candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rDNA |
title_sort | identification and differentiation of the candida
parapsilosis complex species by polymerase chain reaction-restriction
fragment length polymorphism of the internal transcribed spacer region of the
rdna |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830116/ https://www.ncbi.nlm.nih.gov/pubmed/27074256 http://dx.doi.org/10.1590/0074-02760150466 |
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