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Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes

Oxidative stress is harmful to the microbes but also to the host, and may result in bystander damage or death. Because of this, respiratory burst triggered in phagocytes by pathogens is counteracted by production of antioxidative factors. The aim of this work was to examine effectiveness of the latt...

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Autores principales: Homa, J., Stalmach, M., Wilczek, G., Kolaczkowska, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830880/
https://www.ncbi.nlm.nih.gov/pubmed/26922789
http://dx.doi.org/10.1007/s00360-016-0973-5
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author Homa, J.
Stalmach, M.
Wilczek, G.
Kolaczkowska, E.
author_facet Homa, J.
Stalmach, M.
Wilczek, G.
Kolaczkowska, E.
author_sort Homa, J.
collection PubMed
description Oxidative stress is harmful to the microbes but also to the host, and may result in bystander damage or death. Because of this, respiratory burst triggered in phagocytes by pathogens is counteracted by production of antioxidative factors. The aim of this work was to examine effectiveness of the latter system in earthworms Eisenia andrei by induction of reactive oxygen species, lipofuscin and phenoloxidase by natural (LPS, zymosan, Micrococus luteus) and synthetic (phorbol ester, PMA) stimulants. The compounds impaired numbers, viability (increased apoptosis) and composition of coelomocytes, and triggered the antioxidant activity of catalase and selenium-dependent glutathione peroxidase. The natural pathogenic compounds, unlike PMA, strongly activated antioxidative responses that diminished cell apoptosis. Moreover, repeated exposure to the same or different pathogenic compounds did not induce respiratory burst exhausted phenotype showing that coelomocytes are constantly at bay to withstand numerous infections. The current study reveals importance and efficiency of the oxidative–antioxidative systems in annelids but also confirms its evolutionary conservatism and complexity even in lower taxa of the animal kingdom. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00360-016-0973-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-48308802016-04-22 Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes Homa, J. Stalmach, M. Wilczek, G. Kolaczkowska, E. J Comp Physiol B Original Paper Oxidative stress is harmful to the microbes but also to the host, and may result in bystander damage or death. Because of this, respiratory burst triggered in phagocytes by pathogens is counteracted by production of antioxidative factors. The aim of this work was to examine effectiveness of the latter system in earthworms Eisenia andrei by induction of reactive oxygen species, lipofuscin and phenoloxidase by natural (LPS, zymosan, Micrococus luteus) and synthetic (phorbol ester, PMA) stimulants. The compounds impaired numbers, viability (increased apoptosis) and composition of coelomocytes, and triggered the antioxidant activity of catalase and selenium-dependent glutathione peroxidase. The natural pathogenic compounds, unlike PMA, strongly activated antioxidative responses that diminished cell apoptosis. Moreover, repeated exposure to the same or different pathogenic compounds did not induce respiratory burst exhausted phenotype showing that coelomocytes are constantly at bay to withstand numerous infections. The current study reveals importance and efficiency of the oxidative–antioxidative systems in annelids but also confirms its evolutionary conservatism and complexity even in lower taxa of the animal kingdom. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00360-016-0973-5) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-02-27 2016 /pmc/articles/PMC4830880/ /pubmed/26922789 http://dx.doi.org/10.1007/s00360-016-0973-5 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Homa, J.
Stalmach, M.
Wilczek, G.
Kolaczkowska, E.
Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title_full Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title_fullStr Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title_full_unstemmed Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title_short Effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of Eisenia andrei coelomocytes
title_sort effective activation of antioxidant system by immune-relevant factors reversely correlates with apoptosis of eisenia andrei coelomocytes
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830880/
https://www.ncbi.nlm.nih.gov/pubmed/26922789
http://dx.doi.org/10.1007/s00360-016-0973-5
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