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Alternative promoters of Peg3 with maternal specificity

Peg3 (paternally expressed gene 3) is an imprinted gene localized within an evolutionarily conserved 500-kb domain in human chromosome 19q13.4 and mouse proximal chromosome 7. In the current study, we have identified three alternative promoters for mouse Peg3 and one alternative promoter for human P...

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Autores principales: Perera, Bambarendage P. U., Kim, Joomyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830991/
https://www.ncbi.nlm.nih.gov/pubmed/27075691
http://dx.doi.org/10.1038/srep24438
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author Perera, Bambarendage P. U.
Kim, Joomyeong
author_facet Perera, Bambarendage P. U.
Kim, Joomyeong
author_sort Perera, Bambarendage P. U.
collection PubMed
description Peg3 (paternally expressed gene 3) is an imprinted gene localized within an evolutionarily conserved 500-kb domain in human chromosome 19q13.4 and mouse proximal chromosome 7. In the current study, we have identified three alternative promoters for mouse Peg3 and one alternative promoter for human PEG3. These alternative promoters are localized within the 200-kb upstream region of human and mouse PEG3, which is well conserved and thus predicted to harbor several cis-regulatory elements for the PEG3 domain. In the mouse, two of these alternative promoters drive maternal-specific expression of Peg3 specifically in the hypothalamus of the adult brain, while the remaining third promoter drives bi-allelic expression of Peg3 with a paternal bias only in the neonatal-stage brain. In human, an alternative transcript is also detected at relatively very low levels in adult brain and placenta. Overall, the identification of alternative promoters in both mouse and human models suggests that these alternative promoters may be functionally selected features for the PEG3 imprinted domain during mammalian evolution.
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spelling pubmed-48309912016-04-19 Alternative promoters of Peg3 with maternal specificity Perera, Bambarendage P. U. Kim, Joomyeong Sci Rep Article Peg3 (paternally expressed gene 3) is an imprinted gene localized within an evolutionarily conserved 500-kb domain in human chromosome 19q13.4 and mouse proximal chromosome 7. In the current study, we have identified three alternative promoters for mouse Peg3 and one alternative promoter for human PEG3. These alternative promoters are localized within the 200-kb upstream region of human and mouse PEG3, which is well conserved and thus predicted to harbor several cis-regulatory elements for the PEG3 domain. In the mouse, two of these alternative promoters drive maternal-specific expression of Peg3 specifically in the hypothalamus of the adult brain, while the remaining third promoter drives bi-allelic expression of Peg3 with a paternal bias only in the neonatal-stage brain. In human, an alternative transcript is also detected at relatively very low levels in adult brain and placenta. Overall, the identification of alternative promoters in both mouse and human models suggests that these alternative promoters may be functionally selected features for the PEG3 imprinted domain during mammalian evolution. Nature Publishing Group 2016-04-14 /pmc/articles/PMC4830991/ /pubmed/27075691 http://dx.doi.org/10.1038/srep24438 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Perera, Bambarendage P. U.
Kim, Joomyeong
Alternative promoters of Peg3 with maternal specificity
title Alternative promoters of Peg3 with maternal specificity
title_full Alternative promoters of Peg3 with maternal specificity
title_fullStr Alternative promoters of Peg3 with maternal specificity
title_full_unstemmed Alternative promoters of Peg3 with maternal specificity
title_short Alternative promoters of Peg3 with maternal specificity
title_sort alternative promoters of peg3 with maternal specificity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830991/
https://www.ncbi.nlm.nih.gov/pubmed/27075691
http://dx.doi.org/10.1038/srep24438
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