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Detection of Theileria luwenshuni in sheep from Great Britain
BACKGROUND: Theileria spp. are tick-borne protozoan parasites of the Phylum Apicomplexa, Order Piroplasmida that infect a wide range of wild and domestic animals. In Great Britain, Theileria spp. have been reported from livestock associated with transmission by the tick Haemaphysalis punctata. Howev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831081/ https://www.ncbi.nlm.nih.gov/pubmed/27075661 http://dx.doi.org/10.1186/s13071-016-1486-5 |
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author | Phipps, L. Paul Hernández-Triana, Luis M. Goharriz, Hooman Welchman, David Johnson, Nicholas |
author_facet | Phipps, L. Paul Hernández-Triana, Luis M. Goharriz, Hooman Welchman, David Johnson, Nicholas |
author_sort | Phipps, L. Paul |
collection | PubMed |
description | BACKGROUND: Theileria spp. are tick-borne protozoan parasites of the Phylum Apicomplexa, Order Piroplasmida that infect a wide range of wild and domestic animals. In Great Britain, Theileria spp. have been reported from livestock associated with transmission by the tick Haemaphysalis punctata. However, these reports have not been associated with disease. This study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north Kent. FINDINGS: A polymerase chain reaction-reverse line blot assay indicated the presence of Theileria spp. in blood samples from five animals. Subsequent testing with a pan-piroplasm PCR of a larger panel of blood samples detected a piroplasm amplicon in 19 of 21 sheep submitted from the affected flock. Automated sequencing confirmed that these amplicons shared 99–100 % identity with T. luwenshuni. CONCLUSIONS: The clinical and PCR data suggest infection with T. luwenshuni was associated with disease and mortality in this flock. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1486-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4831081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48310812016-04-15 Detection of Theileria luwenshuni in sheep from Great Britain Phipps, L. Paul Hernández-Triana, Luis M. Goharriz, Hooman Welchman, David Johnson, Nicholas Parasit Vectors Short Report BACKGROUND: Theileria spp. are tick-borne protozoan parasites of the Phylum Apicomplexa, Order Piroplasmida that infect a wide range of wild and domestic animals. In Great Britain, Theileria spp. have been reported from livestock associated with transmission by the tick Haemaphysalis punctata. However, these reports have not been associated with disease. This study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north Kent. FINDINGS: A polymerase chain reaction-reverse line blot assay indicated the presence of Theileria spp. in blood samples from five animals. Subsequent testing with a pan-piroplasm PCR of a larger panel of blood samples detected a piroplasm amplicon in 19 of 21 sheep submitted from the affected flock. Automated sequencing confirmed that these amplicons shared 99–100 % identity with T. luwenshuni. CONCLUSIONS: The clinical and PCR data suggest infection with T. luwenshuni was associated with disease and mortality in this flock. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1486-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-13 /pmc/articles/PMC4831081/ /pubmed/27075661 http://dx.doi.org/10.1186/s13071-016-1486-5 Text en © Phipps et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Phipps, L. Paul Hernández-Triana, Luis M. Goharriz, Hooman Welchman, David Johnson, Nicholas Detection of Theileria luwenshuni in sheep from Great Britain |
title | Detection of Theileria luwenshuni in sheep from Great Britain |
title_full | Detection of Theileria luwenshuni in sheep from Great Britain |
title_fullStr | Detection of Theileria luwenshuni in sheep from Great Britain |
title_full_unstemmed | Detection of Theileria luwenshuni in sheep from Great Britain |
title_short | Detection of Theileria luwenshuni in sheep from Great Britain |
title_sort | detection of theileria luwenshuni in sheep from great britain |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831081/ https://www.ncbi.nlm.nih.gov/pubmed/27075661 http://dx.doi.org/10.1186/s13071-016-1486-5 |
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