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ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2

Zeins, the predominent storage proteins in maize endosperm, are encoded by multiple genes and gene families. However, only a few transcriptional factors for zein gene regulation have been functionally characterized. In this study, a MADS-box protein, namely ZmMADS47, was identified as an Opaque2 (O2...

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Autores principales: Qiao, Zhenyi, Qi, Weiwei, Wang, Qian, Feng, Ya’nan, Yang, Qing, Zhang, Nan, Wang, Shanshan, Tang, Yuanping, Song, Rentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831773/
https://www.ncbi.nlm.nih.gov/pubmed/27077660
http://dx.doi.org/10.1371/journal.pgen.1005991
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author Qiao, Zhenyi
Qi, Weiwei
Wang, Qian
Feng, Ya’nan
Yang, Qing
Zhang, Nan
Wang, Shanshan
Tang, Yuanping
Song, Rentao
author_facet Qiao, Zhenyi
Qi, Weiwei
Wang, Qian
Feng, Ya’nan
Yang, Qing
Zhang, Nan
Wang, Shanshan
Tang, Yuanping
Song, Rentao
author_sort Qiao, Zhenyi
collection PubMed
description Zeins, the predominent storage proteins in maize endosperm, are encoded by multiple genes and gene families. However, only a few transcriptional factors for zein gene regulation have been functionally characterized. In this study, a MADS-box protein, namely ZmMADS47, was identified as an Opaque2 (O2) interacting protein via yeast two-hybrid screening. The N-terminal portion of ZmMADS47 contains a nuclear localization signal (NLS), and its C-terminal portion contains a transcriptional activation domain (AD). Interestingly, the transcriptional activation activity is blocked in its full length form, suggesting conformational regulation of the AD. Molecular and RNA-seq analyses of ZmMADS47 RNAi lines revealed down regulation of α-zein and 50-kD γ-zein genes. ZmMADS47 binds the CATGT motif in promoters of these zein genes, but ZmMADS47 alone is not able to transactivate the promoters. However, when both O2 and ZmMADS47 are present, the transactivation of these promoters was greatly enhanced. This enhancement was dependent on the AD function of ZmMADS47 and the interaction between ZmMADS47 and O2, but it was independent from the AD function of O2. Therefore, it appears interaction with O2 activates ZmMADS47 on zein gene promoters.
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spelling pubmed-48317732016-04-22 ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2 Qiao, Zhenyi Qi, Weiwei Wang, Qian Feng, Ya’nan Yang, Qing Zhang, Nan Wang, Shanshan Tang, Yuanping Song, Rentao PLoS Genet Research Article Zeins, the predominent storage proteins in maize endosperm, are encoded by multiple genes and gene families. However, only a few transcriptional factors for zein gene regulation have been functionally characterized. In this study, a MADS-box protein, namely ZmMADS47, was identified as an Opaque2 (O2) interacting protein via yeast two-hybrid screening. The N-terminal portion of ZmMADS47 contains a nuclear localization signal (NLS), and its C-terminal portion contains a transcriptional activation domain (AD). Interestingly, the transcriptional activation activity is blocked in its full length form, suggesting conformational regulation of the AD. Molecular and RNA-seq analyses of ZmMADS47 RNAi lines revealed down regulation of α-zein and 50-kD γ-zein genes. ZmMADS47 binds the CATGT motif in promoters of these zein genes, but ZmMADS47 alone is not able to transactivate the promoters. However, when both O2 and ZmMADS47 are present, the transactivation of these promoters was greatly enhanced. This enhancement was dependent on the AD function of ZmMADS47 and the interaction between ZmMADS47 and O2, but it was independent from the AD function of O2. Therefore, it appears interaction with O2 activates ZmMADS47 on zein gene promoters. Public Library of Science 2016-04-14 /pmc/articles/PMC4831773/ /pubmed/27077660 http://dx.doi.org/10.1371/journal.pgen.1005991 Text en © 2016 Qiao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Qiao, Zhenyi
Qi, Weiwei
Wang, Qian
Feng, Ya’nan
Yang, Qing
Zhang, Nan
Wang, Shanshan
Tang, Yuanping
Song, Rentao
ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title_full ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title_fullStr ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title_full_unstemmed ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title_short ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2
title_sort zmmads47 regulates zein gene transcription through interaction with opaque2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831773/
https://www.ncbi.nlm.nih.gov/pubmed/27077660
http://dx.doi.org/10.1371/journal.pgen.1005991
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