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Control of protein trafficking by reversible masking of transport signals

Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We...

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Autores principales: Abraham, Omer, Gotliv, Karnit, Parnis, Anna, Boncompain, Gaelle, Perez, Franck, Cassel, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831884/
https://www.ncbi.nlm.nih.gov/pubmed/26941332
http://dx.doi.org/10.1091/mbc.E15-07-0472
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author Abraham, Omer
Gotliv, Karnit
Parnis, Anna
Boncompain, Gaelle
Perez, Franck
Cassel, Dan
author_facet Abraham, Omer
Gotliv, Karnit
Parnis, Anna
Boncompain, Gaelle
Perez, Franck
Cassel, Dan
author_sort Abraham, Omer
collection PubMed
description Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We developed an alternative approach for trafficking regulation that we term “controlled unmasking of targeting elements” (CUTE). Regulated trafficking is achieved by reversible masking of the signal that directs the reporter to its target organelle, relying on the streptavidin–biotin system. The targeting signal is generated within or immediately after a 38–amino acid streptavidin-binding peptide (SBP) that is appended to the reporter. The binding of coexpressed streptavidin to SBP causes signal masking, whereas addition of biotin causes complex dissociation and triggers protein transport to the target organelle. We demonstrate the application of this approach to the control of nuclear and peroxisomal protein import and the generation of biotin-dependent trafficking through the endocytic and COPI systems. By simultaneous masking of COPI and endocytic signals, we were able to generate a synthetic pathway for efficient transport of a reporter from the plasma membrane to the endoplasmic reticulum.
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spelling pubmed-48318842016-06-30 Control of protein trafficking by reversible masking of transport signals Abraham, Omer Gotliv, Karnit Parnis, Anna Boncompain, Gaelle Perez, Franck Cassel, Dan Mol Biol Cell Articles Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We developed an alternative approach for trafficking regulation that we term “controlled unmasking of targeting elements” (CUTE). Regulated trafficking is achieved by reversible masking of the signal that directs the reporter to its target organelle, relying on the streptavidin–biotin system. The targeting signal is generated within or immediately after a 38–amino acid streptavidin-binding peptide (SBP) that is appended to the reporter. The binding of coexpressed streptavidin to SBP causes signal masking, whereas addition of biotin causes complex dissociation and triggers protein transport to the target organelle. We demonstrate the application of this approach to the control of nuclear and peroxisomal protein import and the generation of biotin-dependent trafficking through the endocytic and COPI systems. By simultaneous masking of COPI and endocytic signals, we were able to generate a synthetic pathway for efficient transport of a reporter from the plasma membrane to the endoplasmic reticulum. The American Society for Cell Biology 2016-04-15 /pmc/articles/PMC4831884/ /pubmed/26941332 http://dx.doi.org/10.1091/mbc.E15-07-0472 Text en © 2016 Abraham, Gotliv, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Abraham, Omer
Gotliv, Karnit
Parnis, Anna
Boncompain, Gaelle
Perez, Franck
Cassel, Dan
Control of protein trafficking by reversible masking of transport signals
title Control of protein trafficking by reversible masking of transport signals
title_full Control of protein trafficking by reversible masking of transport signals
title_fullStr Control of protein trafficking by reversible masking of transport signals
title_full_unstemmed Control of protein trafficking by reversible masking of transport signals
title_short Control of protein trafficking by reversible masking of transport signals
title_sort control of protein trafficking by reversible masking of transport signals
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831884/
https://www.ncbi.nlm.nih.gov/pubmed/26941332
http://dx.doi.org/10.1091/mbc.E15-07-0472
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