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Control of protein trafficking by reversible masking of transport signals
Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831884/ https://www.ncbi.nlm.nih.gov/pubmed/26941332 http://dx.doi.org/10.1091/mbc.E15-07-0472 |
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author | Abraham, Omer Gotliv, Karnit Parnis, Anna Boncompain, Gaelle Perez, Franck Cassel, Dan |
author_facet | Abraham, Omer Gotliv, Karnit Parnis, Anna Boncompain, Gaelle Perez, Franck Cassel, Dan |
author_sort | Abraham, Omer |
collection | PubMed |
description | Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We developed an alternative approach for trafficking regulation that we term “controlled unmasking of targeting elements” (CUTE). Regulated trafficking is achieved by reversible masking of the signal that directs the reporter to its target organelle, relying on the streptavidin–biotin system. The targeting signal is generated within or immediately after a 38–amino acid streptavidin-binding peptide (SBP) that is appended to the reporter. The binding of coexpressed streptavidin to SBP causes signal masking, whereas addition of biotin causes complex dissociation and triggers protein transport to the target organelle. We demonstrate the application of this approach to the control of nuclear and peroxisomal protein import and the generation of biotin-dependent trafficking through the endocytic and COPI systems. By simultaneous masking of COPI and endocytic signals, we were able to generate a synthetic pathway for efficient transport of a reporter from the plasma membrane to the endoplasmic reticulum. |
format | Online Article Text |
id | pubmed-4831884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-48318842016-06-30 Control of protein trafficking by reversible masking of transport signals Abraham, Omer Gotliv, Karnit Parnis, Anna Boncompain, Gaelle Perez, Franck Cassel, Dan Mol Biol Cell Articles Systems that allow the control of protein traffic between subcellular compartments have been valuable in elucidating trafficking mechanisms. Most current approaches rely on ligand or light-controlled dimerization, which results in either retardation or enhancement of the transport of a reporter. We developed an alternative approach for trafficking regulation that we term “controlled unmasking of targeting elements” (CUTE). Regulated trafficking is achieved by reversible masking of the signal that directs the reporter to its target organelle, relying on the streptavidin–biotin system. The targeting signal is generated within or immediately after a 38–amino acid streptavidin-binding peptide (SBP) that is appended to the reporter. The binding of coexpressed streptavidin to SBP causes signal masking, whereas addition of biotin causes complex dissociation and triggers protein transport to the target organelle. We demonstrate the application of this approach to the control of nuclear and peroxisomal protein import and the generation of biotin-dependent trafficking through the endocytic and COPI systems. By simultaneous masking of COPI and endocytic signals, we were able to generate a synthetic pathway for efficient transport of a reporter from the plasma membrane to the endoplasmic reticulum. The American Society for Cell Biology 2016-04-15 /pmc/articles/PMC4831884/ /pubmed/26941332 http://dx.doi.org/10.1091/mbc.E15-07-0472 Text en © 2016 Abraham, Gotliv, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Abraham, Omer Gotliv, Karnit Parnis, Anna Boncompain, Gaelle Perez, Franck Cassel, Dan Control of protein trafficking by reversible masking of transport signals |
title | Control of protein trafficking by reversible masking of transport signals |
title_full | Control of protein trafficking by reversible masking of transport signals |
title_fullStr | Control of protein trafficking by reversible masking of transport signals |
title_full_unstemmed | Control of protein trafficking by reversible masking of transport signals |
title_short | Control of protein trafficking by reversible masking of transport signals |
title_sort | control of protein trafficking by reversible masking of transport signals |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831884/ https://www.ncbi.nlm.nih.gov/pubmed/26941332 http://dx.doi.org/10.1091/mbc.E15-07-0472 |
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