CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing

The RNA-guided Cas9 nuclease is being widely employed to engineer the genomes of various cells and organisms. Despite the efficient mutagenesis induced by Cas9, off-target effects have raised concerns over the system’s specificity. Recently a “double-nicking” strategy using catalytic mutant Cas9(D10...

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Detalles Bibliográficos
Autores principales: Chiang, Ting-Wei Will, le Sage, Carlos, Larrieu, Delphine, Demir, Mukerrem, Jackson, Stephen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832145/
https://www.ncbi.nlm.nih.gov/pubmed/27079678
http://dx.doi.org/10.1038/srep24356

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832145/
https://www.ncbi.nlm.nih.gov/pubmed/27079678
http://dx.doi.org/10.1038/srep24356

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