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Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus

Sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. The TORC is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. An important feature of haematophagy is...

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Autores principales: Gandara, Ana Caroline P., Oliveira, José Henrique M., Nunes, Rodrigo D., Goncalves, Renata L.S., Dias, Felipe A., Hecht, Fabio, Fernandes, Denise C., Genta, Fernando A., Laurindo, Francisco R.M., Oliveira, Marcus F., Oliveira, Pedro L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832317/
https://www.ncbi.nlm.nih.gov/pubmed/26945025
http://dx.doi.org/10.1042/BSR20160061
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author Gandara, Ana Caroline P.
Oliveira, José Henrique M.
Nunes, Rodrigo D.
Goncalves, Renata L.S.
Dias, Felipe A.
Hecht, Fabio
Fernandes, Denise C.
Genta, Fernando A.
Laurindo, Francisco R.M.
Oliveira, Marcus F.
Oliveira, Pedro L.
author_facet Gandara, Ana Caroline P.
Oliveira, José Henrique M.
Nunes, Rodrigo D.
Goncalves, Renata L.S.
Dias, Felipe A.
Hecht, Fabio
Fernandes, Denise C.
Genta, Fernando A.
Laurindo, Francisco R.M.
Oliveira, Marcus F.
Oliveira, Pedro L.
author_sort Gandara, Ana Caroline P.
collection PubMed
description Sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. The TORC is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. An important feature of haematophagy is that huge amounts of blood are ingested in a single meal, which results in the release of large quantities of amino acids, together with the haemoglobin prosthetic group, haem, which decomposes hydroperoxides and propagates oxygen-derived free radicals. Our previous studies demonstrated that reactive oxygen species (ROS) levels were diminished in the mitochondria and midgut of the Dengue fever mosquito, Aedes aegypti, immediately after a blood meal. We proposed that this mechanism serves to avoid oxidative damage that would otherwise be induced by haem following a blood meal. Studies also performed in mosquitoes have shown that blood or amino acids controls protein synthesis through TORC activation. It was already proposed, in different models, a link between ROS and TOR, however, little is known about TOR signalling in insect midgut nor about the involvement of ROS in this pathway. Here, we studied the effect of a blood meal on ROS production in the midgut of Rhodnius prolixus. We observed that blood meal amino acids decreased ROS levels in the R. prolixus midgut immediately after feeding, via lowering mitochondrial superoxide production and involving the amino acid-sensing TORC pathway.
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spelling pubmed-48323172016-04-25 Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus Gandara, Ana Caroline P. Oliveira, José Henrique M. Nunes, Rodrigo D. Goncalves, Renata L.S. Dias, Felipe A. Hecht, Fabio Fernandes, Denise C. Genta, Fernando A. Laurindo, Francisco R.M. Oliveira, Marcus F. Oliveira, Pedro L. Biosci Rep Original Papers Sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. The TORC is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. An important feature of haematophagy is that huge amounts of blood are ingested in a single meal, which results in the release of large quantities of amino acids, together with the haemoglobin prosthetic group, haem, which decomposes hydroperoxides and propagates oxygen-derived free radicals. Our previous studies demonstrated that reactive oxygen species (ROS) levels were diminished in the mitochondria and midgut of the Dengue fever mosquito, Aedes aegypti, immediately after a blood meal. We proposed that this mechanism serves to avoid oxidative damage that would otherwise be induced by haem following a blood meal. Studies also performed in mosquitoes have shown that blood or amino acids controls protein synthesis through TORC activation. It was already proposed, in different models, a link between ROS and TOR, however, little is known about TOR signalling in insect midgut nor about the involvement of ROS in this pathway. Here, we studied the effect of a blood meal on ROS production in the midgut of Rhodnius prolixus. We observed that blood meal amino acids decreased ROS levels in the R. prolixus midgut immediately after feeding, via lowering mitochondrial superoxide production and involving the amino acid-sensing TORC pathway. Portland Press Ltd. 2016-04-15 /pmc/articles/PMC4832317/ /pubmed/26945025 http://dx.doi.org/10.1042/BSR20160061 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Gandara, Ana Caroline P.
Oliveira, José Henrique M.
Nunes, Rodrigo D.
Goncalves, Renata L.S.
Dias, Felipe A.
Hecht, Fabio
Fernandes, Denise C.
Genta, Fernando A.
Laurindo, Francisco R.M.
Oliveira, Marcus F.
Oliveira, Pedro L.
Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title_full Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title_fullStr Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title_full_unstemmed Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title_short Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus
title_sort amino acids trigger down-regulation of superoxide via torc pathway in the midgut of rhodnius prolixus
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832317/
https://www.ncbi.nlm.nih.gov/pubmed/26945025
http://dx.doi.org/10.1042/BSR20160061
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