Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line

The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental condition...

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Detalles Bibliográficos
Autores principales: Presley, Chaela S., Abidi, Ammaar H., Moore, Bob M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833130/
https://www.ncbi.nlm.nih.gov/pubmed/27115025
http://dx.doi.org/10.1016/j.dib.2016.03.086
Descripción
Sumario:The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of G(i/o).

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