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Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line

The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental condition...

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Detalles Bibliográficos
Autores principales: Presley, Chaela S., Abidi, Ammaar H., Moore, Bob M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833130/
https://www.ncbi.nlm.nih.gov/pubmed/27115025
http://dx.doi.org/10.1016/j.dib.2016.03.086
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author Presley, Chaela S.
Abidi, Ammaar H.
Moore, Bob M.
author_facet Presley, Chaela S.
Abidi, Ammaar H.
Moore, Bob M.
author_sort Presley, Chaela S.
collection PubMed
description The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of G(i/o).
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spelling pubmed-48331302016-04-25 Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line Presley, Chaela S. Abidi, Ammaar H. Moore, Bob M. Data Brief Data Article The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of G(i/o). Elsevier 2016-04-01 /pmc/articles/PMC4833130/ /pubmed/27115025 http://dx.doi.org/10.1016/j.dib.2016.03.086 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Presley, Chaela S.
Abidi, Ammaar H.
Moore, Bob M.
Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_full Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_fullStr Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_full_unstemmed Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_short Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_sort optimization of camp fluorescence dataset from actone cannabinoid receptor 1 cell line
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833130/
https://www.ncbi.nlm.nih.gov/pubmed/27115025
http://dx.doi.org/10.1016/j.dib.2016.03.086
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