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Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental condition...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833130/ https://www.ncbi.nlm.nih.gov/pubmed/27115025 http://dx.doi.org/10.1016/j.dib.2016.03.086 |
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author | Presley, Chaela S. Abidi, Ammaar H. Moore, Bob M. |
author_facet | Presley, Chaela S. Abidi, Ammaar H. Moore, Bob M. |
author_sort | Presley, Chaela S. |
collection | PubMed |
description | The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of G(i/o). |
format | Online Article Text |
id | pubmed-4833130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48331302016-04-25 Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line Presley, Chaela S. Abidi, Ammaar H. Moore, Bob M. Data Brief Data Article The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of G(i/o). Elsevier 2016-04-01 /pmc/articles/PMC4833130/ /pubmed/27115025 http://dx.doi.org/10.1016/j.dib.2016.03.086 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Presley, Chaela S. Abidi, Ammaar H. Moore, Bob M. Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title | Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title_full | Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title_fullStr | Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title_full_unstemmed | Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title_short | Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line |
title_sort | optimization of camp fluorescence dataset from actone cannabinoid receptor 1 cell line |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833130/ https://www.ncbi.nlm.nih.gov/pubmed/27115025 http://dx.doi.org/10.1016/j.dib.2016.03.086 |
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