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Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells
ZSCAN4 is a DNA-binding protein that functions for telomere elongation and genomic stability. In vivo, it is specifically expressed at the two-cell stage during mouse development. In vitro, it is transiently expressed in mouse embryonic stem cells (ESCs), only in 5% of the population at one time. He...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834046/ https://www.ncbi.nlm.nih.gov/pubmed/26997646 http://dx.doi.org/10.1016/j.stemcr.2016.02.010 |
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author | Nakai-Futatsugi, Yoko Niwa, Hitoshi |
author_facet | Nakai-Futatsugi, Yoko Niwa, Hitoshi |
author_sort | Nakai-Futatsugi, Yoko |
collection | PubMed |
description | ZSCAN4 is a DNA-binding protein that functions for telomere elongation and genomic stability. In vivo, it is specifically expressed at the two-cell stage during mouse development. In vitro, it is transiently expressed in mouse embryonic stem cells (ESCs), only in 5% of the population at one time. Here we attempted to elucidate when, under what circumstances, Zscan4 is activated in ESCs. Using live cell imaging, we monitored the activity of Zscan4 together with the pluripotency marker Rex1. The lengths of the cell cycles in ESCs were diverse. Longer cell cycles were accompanied by shorter telomeres and higher activation of Zscan4. Since activation of Zscan4 is involved in telomere elongation, we speculate that the extended cell cycles accompanied by Zscan4 activation reflect the time for telomere recovery. Rex1 and Zscan4 did not show any correlation. Taken together, we propose that Zscan4 is activated to recover shortened telomeres during extended cell cycles, irrespective of the pluripotent status. |
format | Online Article Text |
id | pubmed-4834046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48340462016-04-27 Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells Nakai-Futatsugi, Yoko Niwa, Hitoshi Stem Cell Reports Article ZSCAN4 is a DNA-binding protein that functions for telomere elongation and genomic stability. In vivo, it is specifically expressed at the two-cell stage during mouse development. In vitro, it is transiently expressed in mouse embryonic stem cells (ESCs), only in 5% of the population at one time. Here we attempted to elucidate when, under what circumstances, Zscan4 is activated in ESCs. Using live cell imaging, we monitored the activity of Zscan4 together with the pluripotency marker Rex1. The lengths of the cell cycles in ESCs were diverse. Longer cell cycles were accompanied by shorter telomeres and higher activation of Zscan4. Since activation of Zscan4 is involved in telomere elongation, we speculate that the extended cell cycles accompanied by Zscan4 activation reflect the time for telomere recovery. Rex1 and Zscan4 did not show any correlation. Taken together, we propose that Zscan4 is activated to recover shortened telomeres during extended cell cycles, irrespective of the pluripotent status. Elsevier 2016-03-17 /pmc/articles/PMC4834046/ /pubmed/26997646 http://dx.doi.org/10.1016/j.stemcr.2016.02.010 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nakai-Futatsugi, Yoko Niwa, Hitoshi Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title | Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title_full | Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title_fullStr | Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title_full_unstemmed | Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title_short | Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells |
title_sort | zscan4 is activated after telomere shortening in mouse embryonic stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834046/ https://www.ncbi.nlm.nih.gov/pubmed/26997646 http://dx.doi.org/10.1016/j.stemcr.2016.02.010 |
work_keys_str_mv | AT nakaifutatsugiyoko zscan4isactivatedaftertelomereshorteninginmouseembryonicstemcells AT niwahitoshi zscan4isactivatedaftertelomereshorteninginmouseembryonicstemcells |