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Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells
Here, we show that the lysophosphatidic acid receptor 1 (LPA(1)) is expressed by a defined population of type 1 stem cells and type 2a precursor cells in the adult mouse dentate gyrus. LPA(1), in contrast to Nestin, also marks the quiescent stem cell population. Combining LPA(1)-GFP with EGFR and pr...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834054/ https://www.ncbi.nlm.nih.gov/pubmed/27050949 http://dx.doi.org/10.1016/j.stemcr.2016.03.002 |
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author | Walker, Tara L. Overall, Rupert W. Vogler, Steffen Sykes, Alex M. Ruhwald, Susann Lasse, Daniela Ichwan, Muhammad Fabel, Klaus Kempermann, Gerd |
author_facet | Walker, Tara L. Overall, Rupert W. Vogler, Steffen Sykes, Alex M. Ruhwald, Susann Lasse, Daniela Ichwan, Muhammad Fabel, Klaus Kempermann, Gerd |
author_sort | Walker, Tara L. |
collection | PubMed |
description | Here, we show that the lysophosphatidic acid receptor 1 (LPA(1)) is expressed by a defined population of type 1 stem cells and type 2a precursor cells in the adult mouse dentate gyrus. LPA(1), in contrast to Nestin, also marks the quiescent stem cell population. Combining LPA(1)-GFP with EGFR and prominin-1 expression, we have enabled the prospective separation of both proliferative and non-proliferative precursor cell populations. Transcriptional profiling of the isolated proliferative precursor cells suggested immune mechanisms and cytokine signaling as molecular regulators of adult hippocampal precursor cell proliferation. In addition to LPA(1) being a marker of this important stem cell population, we also show that the corresponding ligand LPA is directly involved in the regulation of adult hippocampal precursor cell proliferation and neurogenesis, an effect that can be attributed to LPA signaling via the AKT and MAPK pathways. |
format | Online Article Text |
id | pubmed-4834054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48340542016-04-27 Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells Walker, Tara L. Overall, Rupert W. Vogler, Steffen Sykes, Alex M. Ruhwald, Susann Lasse, Daniela Ichwan, Muhammad Fabel, Klaus Kempermann, Gerd Stem Cell Reports Article Here, we show that the lysophosphatidic acid receptor 1 (LPA(1)) is expressed by a defined population of type 1 stem cells and type 2a precursor cells in the adult mouse dentate gyrus. LPA(1), in contrast to Nestin, also marks the quiescent stem cell population. Combining LPA(1)-GFP with EGFR and prominin-1 expression, we have enabled the prospective separation of both proliferative and non-proliferative precursor cell populations. Transcriptional profiling of the isolated proliferative precursor cells suggested immune mechanisms and cytokine signaling as molecular regulators of adult hippocampal precursor cell proliferation. In addition to LPA(1) being a marker of this important stem cell population, we also show that the corresponding ligand LPA is directly involved in the regulation of adult hippocampal precursor cell proliferation and neurogenesis, an effect that can be attributed to LPA signaling via the AKT and MAPK pathways. Elsevier 2016-03-31 /pmc/articles/PMC4834054/ /pubmed/27050949 http://dx.doi.org/10.1016/j.stemcr.2016.03.002 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Walker, Tara L. Overall, Rupert W. Vogler, Steffen Sykes, Alex M. Ruhwald, Susann Lasse, Daniela Ichwan, Muhammad Fabel, Klaus Kempermann, Gerd Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title | Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title_full | Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title_fullStr | Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title_full_unstemmed | Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title_short | Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells |
title_sort | lysophosphatidic acid receptor is a functional marker of adult hippocampal precursor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834054/ https://www.ncbi.nlm.nih.gov/pubmed/27050949 http://dx.doi.org/10.1016/j.stemcr.2016.03.002 |
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