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Hyperbaric oxygen therapy improves colorectal anastomotic healing

PURPOSE: Hyperbaric oxygen treatment (HBOT) has been found to improve the healing of poorly oxygenated tissues. This study aimed to investigate the influence of HBOT on the healing in ischemic colorectal anastomosis. METHODS: Forty Wistar rats were randomly divided into a treatment group that receiv...

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Autores principales: Boersema, G. S. A., Wu, Z., Kroese, L. F., Vennix, S., Bastiaansen-Jenniskens, Y. M., van Neck, J. W., Lam, K. H., Kleinrensink, G. J., Jeekel, J., Lange, J. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834105/
https://www.ncbi.nlm.nih.gov/pubmed/27041554
http://dx.doi.org/10.1007/s00384-016-2573-y
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author Boersema, G. S. A.
Wu, Z.
Kroese, L. F.
Vennix, S.
Bastiaansen-Jenniskens, Y. M.
van Neck, J. W.
Lam, K. H.
Kleinrensink, G. J.
Jeekel, J.
Lange, J. F.
author_facet Boersema, G. S. A.
Wu, Z.
Kroese, L. F.
Vennix, S.
Bastiaansen-Jenniskens, Y. M.
van Neck, J. W.
Lam, K. H.
Kleinrensink, G. J.
Jeekel, J.
Lange, J. F.
author_sort Boersema, G. S. A.
collection PubMed
description PURPOSE: Hyperbaric oxygen treatment (HBOT) has been found to improve the healing of poorly oxygenated tissues. This study aimed to investigate the influence of HBOT on the healing in ischemic colorectal anastomosis. METHODS: Forty Wistar rats were randomly divided into a treatment group that received HBOT for 10 consecutive days (7 days before and 3 days after surgery), or in a control group, which did not receive the therapy. Colectomy with an ischemic anastomosis was performed in all rats. In each group, the rats were followed for 3 or 7 days after surgery to determine the influence of HBOT on anastomotic healing. RESULTS: Five rats from each group died during follow-up. No anastomotic dehiscence was seen in the HBOT group, compared to 37.5 % and 28.6 % dehiscence in the control group on postoperative day (POD) 3 and 7, respectively. The HBOT group had a significantly higher bursting pressure (130.9 ± 17.0 mmHg) than the control group (88.4 ± 46.7 mmHg; p = 0.03) on POD 3. On POD 3 and POD 7, the adhesion severity was significantly higher in the control groups than in the HBOT groups (p < 0.005). Kidney function (creatinine level) of the HBOT group was significantly better than of the control group on POD 7 (p = 0.001). Interestingly, a significantly higher number of CD206+ cells (marker for type 2 macrophages) was observed in the HBOT group at the anastomotic area on POD 3. CONCLUSION: Hyperbaric oxygen enhanced the healing of ischemic anastomoses in rats and improved the postoperative kidney function. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00384-016-2573-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-48341052016-04-26 Hyperbaric oxygen therapy improves colorectal anastomotic healing Boersema, G. S. A. Wu, Z. Kroese, L. F. Vennix, S. Bastiaansen-Jenniskens, Y. M. van Neck, J. W. Lam, K. H. Kleinrensink, G. J. Jeekel, J. Lange, J. F. Int J Colorectal Dis Original Article PURPOSE: Hyperbaric oxygen treatment (HBOT) has been found to improve the healing of poorly oxygenated tissues. This study aimed to investigate the influence of HBOT on the healing in ischemic colorectal anastomosis. METHODS: Forty Wistar rats were randomly divided into a treatment group that received HBOT for 10 consecutive days (7 days before and 3 days after surgery), or in a control group, which did not receive the therapy. Colectomy with an ischemic anastomosis was performed in all rats. In each group, the rats were followed for 3 or 7 days after surgery to determine the influence of HBOT on anastomotic healing. RESULTS: Five rats from each group died during follow-up. No anastomotic dehiscence was seen in the HBOT group, compared to 37.5 % and 28.6 % dehiscence in the control group on postoperative day (POD) 3 and 7, respectively. The HBOT group had a significantly higher bursting pressure (130.9 ± 17.0 mmHg) than the control group (88.4 ± 46.7 mmHg; p = 0.03) on POD 3. On POD 3 and POD 7, the adhesion severity was significantly higher in the control groups than in the HBOT groups (p < 0.005). Kidney function (creatinine level) of the HBOT group was significantly better than of the control group on POD 7 (p = 0.001). Interestingly, a significantly higher number of CD206+ cells (marker for type 2 macrophages) was observed in the HBOT group at the anastomotic area on POD 3. CONCLUSION: Hyperbaric oxygen enhanced the healing of ischemic anastomoses in rats and improved the postoperative kidney function. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00384-016-2573-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-04-04 2016 /pmc/articles/PMC4834105/ /pubmed/27041554 http://dx.doi.org/10.1007/s00384-016-2573-y Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Boersema, G. S. A.
Wu, Z.
Kroese, L. F.
Vennix, S.
Bastiaansen-Jenniskens, Y. M.
van Neck, J. W.
Lam, K. H.
Kleinrensink, G. J.
Jeekel, J.
Lange, J. F.
Hyperbaric oxygen therapy improves colorectal anastomotic healing
title Hyperbaric oxygen therapy improves colorectal anastomotic healing
title_full Hyperbaric oxygen therapy improves colorectal anastomotic healing
title_fullStr Hyperbaric oxygen therapy improves colorectal anastomotic healing
title_full_unstemmed Hyperbaric oxygen therapy improves colorectal anastomotic healing
title_short Hyperbaric oxygen therapy improves colorectal anastomotic healing
title_sort hyperbaric oxygen therapy improves colorectal anastomotic healing
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834105/
https://www.ncbi.nlm.nih.gov/pubmed/27041554
http://dx.doi.org/10.1007/s00384-016-2573-y
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