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Protective effect of crocin on gentamicin-induced nephrotoxicity in rats

OBJECTIVE(S): Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. MATERIALS...

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Autores principales: Yarijani, Zeynab Mohamadi, Najafi, Houshang, Hamid Madani, Seyed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834125/
https://www.ncbi.nlm.nih.gov/pubmed/27114805
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author Yarijani, Zeynab Mohamadi
Najafi, Houshang
Hamid Madani, Seyed
author_facet Yarijani, Zeynab Mohamadi
Najafi, Houshang
Hamid Madani, Seyed
author_sort Yarijani, Zeynab Mohamadi
collection PubMed
description OBJECTIVE(S): Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS: Thirty two male Wistar rats received gentamicin (100 mg/kg, IP), with or without crocin (100 mg/kg, IP) for seven consecutive days. Plasma creatinine and urea-nitrogen concentrations, oxidative stress and histopathological changes of kidney tissues were monitored. RESULTS: Administration of gentamicin resulted in significant increases in plasma creatinine and urea-nitrogen concentrations and renal tissue malondialdehyde (MDA) level, and a decrease in the renal tissue ferric reducing/antioxidant power (FRAP) level. Crocin decreased plasma creatinine and urea-nitrogen concentrations and tissue MDA level, but increased the level of tissue FRAP. In addition, gentamicin led to cellular damages including glomerular atrophy, cellular desquamation, tubular necrosis and fibrosis, epithelial oedema of proximal tubules, perivascular edema, vascular congestion and intra-tubular proteinaceous casts, all of which were partially recovered by crocin. CONCLUSION: Crocin has protective effects against functional disturbances, oxidative stress and tissue damages induced by gentamicin.
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spelling pubmed-48341252016-04-25 Protective effect of crocin on gentamicin-induced nephrotoxicity in rats Yarijani, Zeynab Mohamadi Najafi, Houshang Hamid Madani, Seyed Iran J Basic Med Sci Short Communication OBJECTIVE(S): Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS: Thirty two male Wistar rats received gentamicin (100 mg/kg, IP), with or without crocin (100 mg/kg, IP) for seven consecutive days. Plasma creatinine and urea-nitrogen concentrations, oxidative stress and histopathological changes of kidney tissues were monitored. RESULTS: Administration of gentamicin resulted in significant increases in plasma creatinine and urea-nitrogen concentrations and renal tissue malondialdehyde (MDA) level, and a decrease in the renal tissue ferric reducing/antioxidant power (FRAP) level. Crocin decreased plasma creatinine and urea-nitrogen concentrations and tissue MDA level, but increased the level of tissue FRAP. In addition, gentamicin led to cellular damages including glomerular atrophy, cellular desquamation, tubular necrosis and fibrosis, epithelial oedema of proximal tubules, perivascular edema, vascular congestion and intra-tubular proteinaceous casts, all of which were partially recovered by crocin. CONCLUSION: Crocin has protective effects against functional disturbances, oxidative stress and tissue damages induced by gentamicin. Mashhad University of Medical Sciences 2016-03 /pmc/articles/PMC4834125/ /pubmed/27114805 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Yarijani, Zeynab Mohamadi
Najafi, Houshang
Hamid Madani, Seyed
Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title_full Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title_fullStr Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title_full_unstemmed Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title_short Protective effect of crocin on gentamicin-induced nephrotoxicity in rats
title_sort protective effect of crocin on gentamicin-induced nephrotoxicity in rats
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834125/
https://www.ncbi.nlm.nih.gov/pubmed/27114805
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