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Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters

Photosynthetic picoeukaryotes are significant contributors to marine primary productivity. Associations between marine bacterioplankton and picoeukaryotes frequently occur and can have large biogeochemical impacts. We used flow cytometry to sort cells from seawater to identify non-eukaryotic phyloty...

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Autores principales: Farnelid, Hanna M., Turk-Kubo, Kendra A., Zehr, Jonathan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834442/
https://www.ncbi.nlm.nih.gov/pubmed/27148165
http://dx.doi.org/10.3389/fmicb.2016.00339
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author Farnelid, Hanna M.
Turk-Kubo, Kendra A.
Zehr, Jonathan P.
author_facet Farnelid, Hanna M.
Turk-Kubo, Kendra A.
Zehr, Jonathan P.
author_sort Farnelid, Hanna M.
collection PubMed
description Photosynthetic picoeukaryotes are significant contributors to marine primary productivity. Associations between marine bacterioplankton and picoeukaryotes frequently occur and can have large biogeochemical impacts. We used flow cytometry to sort cells from seawater to identify non-eukaryotic phylotypes that are associated with photosynthetic picoeukaryotes. Samples were collected at the Santa Cruz wharf on Monterey Bay, CA, USA during summer and fall, 2014. The phylogeny of associated microbes was assessed through 16S rRNA gene amplicon clone and Illumina MiSeq libraries. The most frequently detected bacterioplankton phyla within the photosynthetic picoeukaryote sorts were Proteobacteria (Alphaproteobacteria and Gammaproteobacteria) and Bacteroidetes. Intriguingly, the presence of free-living bacterial genera in the photosynthetic picoeukaryote sorts could suggest that some of the photosynthetic picoeukaryotes were mixotrophs. However, the occurrence of bacterial sequences, which were not prevalent in the corresponding bulk seawater samples, indicates that there was also a selection for specific OTUs in association with photosynthetic picoeukaryotes suggesting specific functional associations. The results show that diverse bacterial phylotypes are found in association with photosynthetic picoeukaryotes. Taxonomic identification of these associations is a prerequisite for further characterizing and to elucidate their metabolic pathways and ecological functions.
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spelling pubmed-48344422016-05-04 Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters Farnelid, Hanna M. Turk-Kubo, Kendra A. Zehr, Jonathan P. Front Microbiol Microbiology Photosynthetic picoeukaryotes are significant contributors to marine primary productivity. Associations between marine bacterioplankton and picoeukaryotes frequently occur and can have large biogeochemical impacts. We used flow cytometry to sort cells from seawater to identify non-eukaryotic phylotypes that are associated with photosynthetic picoeukaryotes. Samples were collected at the Santa Cruz wharf on Monterey Bay, CA, USA during summer and fall, 2014. The phylogeny of associated microbes was assessed through 16S rRNA gene amplicon clone and Illumina MiSeq libraries. The most frequently detected bacterioplankton phyla within the photosynthetic picoeukaryote sorts were Proteobacteria (Alphaproteobacteria and Gammaproteobacteria) and Bacteroidetes. Intriguingly, the presence of free-living bacterial genera in the photosynthetic picoeukaryote sorts could suggest that some of the photosynthetic picoeukaryotes were mixotrophs. However, the occurrence of bacterial sequences, which were not prevalent in the corresponding bulk seawater samples, indicates that there was also a selection for specific OTUs in association with photosynthetic picoeukaryotes suggesting specific functional associations. The results show that diverse bacterial phylotypes are found in association with photosynthetic picoeukaryotes. Taxonomic identification of these associations is a prerequisite for further characterizing and to elucidate their metabolic pathways and ecological functions. Frontiers Media S.A. 2016-03-22 /pmc/articles/PMC4834442/ /pubmed/27148165 http://dx.doi.org/10.3389/fmicb.2016.00339 Text en Copyright © 2016 Farnelid, Turk-Kubo and Zehr. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Farnelid, Hanna M.
Turk-Kubo, Kendra A.
Zehr, Jonathan P.
Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title_full Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title_fullStr Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title_full_unstemmed Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title_short Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
title_sort identification of associations between bacterioplankton and photosynthetic picoeukaryotes in coastal waters
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834442/
https://www.ncbi.nlm.nih.gov/pubmed/27148165
http://dx.doi.org/10.3389/fmicb.2016.00339
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