Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line

Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK...

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Autores principales: Haridas, Parvathi, McGovern, Jacqui A., Kashyap, Abhishek S., McElwain, D. L. Sean, Simpson, Matthew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834532/
https://www.ncbi.nlm.nih.gov/pubmed/27087056
http://dx.doi.org/10.1038/srep24569
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author Haridas, Parvathi
McGovern, Jacqui A.
Kashyap, Abhishek S.
McElwain, D. L. Sean
Simpson, Matthew J.
author_facet Haridas, Parvathi
McGovern, Jacqui A.
Kashyap, Abhishek S.
McElwain, D. L. Sean
Simpson, Matthew J.
author_sort Haridas, Parvathi
collection PubMed
description Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care.
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spelling pubmed-48345322016-04-27 Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line Haridas, Parvathi McGovern, Jacqui A. Kashyap, Abhishek S. McElwain, D. L. Sean Simpson, Matthew J. Sci Rep Article Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care. Nature Publishing Group 2016-04-18 /pmc/articles/PMC4834532/ /pubmed/27087056 http://dx.doi.org/10.1038/srep24569 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Haridas, Parvathi
McGovern, Jacqui A.
Kashyap, Abhishek S.
McElwain, D. L. Sean
Simpson, Matthew J.
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title_full Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title_fullStr Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title_full_unstemmed Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title_short Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
title_sort standard melanoma-associated markers do not identify the mm127 metastatic melanoma cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834532/
https://www.ncbi.nlm.nih.gov/pubmed/27087056
http://dx.doi.org/10.1038/srep24569
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