Intensity interrogation near cutoff resonance for label-free cellular profiling

We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substr...

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Detalles Bibliográficos
Autores principales: Nazirizadeh, Yousef, Behrends, Volker, Prósz, Aurél, Orgovan, Norbert, Horvath, Robert, Ferrie, Ann M., Fang, Ye, Selhuber-Unkel, Christine, Gerken, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834563/
https://www.ncbi.nlm.nih.gov/pubmed/27086879
http://dx.doi.org/10.1038/srep24685
Descripción
Sumario:We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

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