Intensity interrogation near cutoff resonance for label-free cellular profiling

We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substr...

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Autores principales: Nazirizadeh, Yousef, Behrends, Volker, Prósz, Aurél, Orgovan, Norbert, Horvath, Robert, Ferrie, Ann M., Fang, Ye, Selhuber-Unkel, Christine, Gerken, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834563/
https://www.ncbi.nlm.nih.gov/pubmed/27086879
http://dx.doi.org/10.1038/srep24685
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author Nazirizadeh, Yousef
Behrends, Volker
Prósz, Aurél
Orgovan, Norbert
Horvath, Robert
Ferrie, Ann M.
Fang, Ye
Selhuber-Unkel, Christine
Gerken, Martina
author_facet Nazirizadeh, Yousef
Behrends, Volker
Prósz, Aurél
Orgovan, Norbert
Horvath, Robert
Ferrie, Ann M.
Fang, Ye
Selhuber-Unkel, Christine
Gerken, Martina
author_sort Nazirizadeh, Yousef
collection PubMed
description We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.
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spelling pubmed-48345632016-04-27 Intensity interrogation near cutoff resonance for label-free cellular profiling Nazirizadeh, Yousef Behrends, Volker Prósz, Aurél Orgovan, Norbert Horvath, Robert Ferrie, Ann M. Fang, Ye Selhuber-Unkel, Christine Gerken, Martina Sci Rep Article We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems. Nature Publishing Group 2016-04-18 /pmc/articles/PMC4834563/ /pubmed/27086879 http://dx.doi.org/10.1038/srep24685 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nazirizadeh, Yousef
Behrends, Volker
Prósz, Aurél
Orgovan, Norbert
Horvath, Robert
Ferrie, Ann M.
Fang, Ye
Selhuber-Unkel, Christine
Gerken, Martina
Intensity interrogation near cutoff resonance for label-free cellular profiling
title Intensity interrogation near cutoff resonance for label-free cellular profiling
title_full Intensity interrogation near cutoff resonance for label-free cellular profiling
title_fullStr Intensity interrogation near cutoff resonance for label-free cellular profiling
title_full_unstemmed Intensity interrogation near cutoff resonance for label-free cellular profiling
title_short Intensity interrogation near cutoff resonance for label-free cellular profiling
title_sort intensity interrogation near cutoff resonance for label-free cellular profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834563/
https://www.ncbi.nlm.nih.gov/pubmed/27086879
http://dx.doi.org/10.1038/srep24685
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