A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells

The rapid adoption of gene editing tools such as CRISPRs and TALENs for research and eventually therapeutics necessitates assays that can rapidly detect and quantitate the desired alterations. Currently, the most commonly used assay employs “mismatch nucleases” T7E1 or “Surveyor” that recognize and...

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Detalles Bibliográficos
Autores principales: Findlay, Scott D., Vincent, Krista M., Berman, Jennifer R., Postovit, Lynne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835065/
https://www.ncbi.nlm.nih.gov/pubmed/27089539
http://dx.doi.org/10.1371/journal.pone.0153901

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835065/
https://www.ncbi.nlm.nih.gov/pubmed/27089539
http://dx.doi.org/10.1371/journal.pone.0153901

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