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A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells
The rapid adoption of gene editing tools such as CRISPRs and TALENs for research and eventually therapeutics necessitates assays that can rapidly detect and quantitate the desired alterations. Currently, the most commonly used assay employs “mismatch nucleases” T7E1 or “Surveyor” that recognize and...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835065/ https://www.ncbi.nlm.nih.gov/pubmed/27089539 http://dx.doi.org/10.1371/journal.pone.0153901 |